Date published: 2025-12-23

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Triton X-100 (CAS 9002-93-1)

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Application:
Triton X-100 is a non-ionic surfactant and emulsifier widely used for solubilizing membrane proteins
CAS Number:
9002-93-1
Molecular Weight:
646.86
Molecular Formula:
C34H62O11
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Available in US only.
* Refer to Certificate of Analysis for lot specific data.

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Triton X-100 is a non-ionic surfactant that belongs to a group of related compounds known as alkylphenol ethoxylates. It is also an emulsifier, widely used for the solubilization of membranes and recovery of membrane components under mild, non-denaturing conditions. It is used in the enhancement of film porosity in conducting polymers. Composed of a hydrophobic polyoxyethylene group, which allows it to decrease surface tensions, Triton X-100 is used in biochemistry and molecular biology for lysing cells to extract protein and other cellular components. Its chemical structure is characterized by an aromatic ring (phenol) with a polyethylene oxide chain and an isooctyl phenyl group, facilitating its role as a detergent that can solubilize proteins and disrupt lipid-lipid and lipid-protein interactions.


Triton X-100 (CAS 9002-93-1) References

  1. Nondetergent isolation of rafts.  |  Shah, MB. and Sehgal, PB. 2007. Methods Mol Biol. 398: 21-8. PMID: 18214371
  2. The isolation of detergent-resistant lipid rafts for two-dimensional electrophoresis.  |  Kim, KB., et al. 2008. Methods Mol Biol. 424: 413-22. PMID: 18369879
  3. Mammalian long-chain acyl-CoA synthetases.  |  Soupene, E. and Kuypers, FA. 2008. Exp Biol Med (Maywood). 233: 507-21. PMID: 18375835
  4. Permeabilization of cell membranes.  |  Jamur, MC. and Oliver, C. 2010. Methods Mol Biol. 588: 63-6. PMID: 20012820
  5. Identification and characterization of a Triton X-100 replacement for virus inactivation.  |  Luo, W., et al. 2020. Biotechnol Prog. 36: e3036. PMID: 32533632
  6. Triton X-100-Modified Adenosine Triphosphate-Responsive siRNA Delivery Agent for Antitumor Therapy.  |  Fan, X., et al. 2020. Mol Pharm. 17: 3696-3708. PMID: 32803981
  7. The Effect of Residual Triton X-100 on Structural Stability and Infection Activity of Adenovirus Particles.  |  Ma, J., et al. 2020. Mol Ther Methods Clin Dev. 19: 35-46. PMID: 32995358
  8. Changes in the aquatic ecotoxicological effects of Triton X-100 after UV photodegradation.  |  Jho, EH., et al. 2021. Environ Sci Pollut Res Int. 28: 11224-11232. PMID: 33113057
  9. Triton X-100 improves co-production of β-1,3-D-glucan and pullulan by Aureobasidium pullulans.  |  Wang, GL., et al. 2020. Appl Microbiol Biotechnol. 104: 10685-10696. PMID: 33170326
  10. Application Value of Triton X-100 to Modified Hodge Test and Carbapenem Inactivation Method in the Detection of Acinetobacter baumannii Carbapenemase.  |  Fan, S., et al. 2020. Infect Drug Resist. 13: 4283-4288. PMID: 33262622
  11. Triton X-100 supplementation regulates growth and secondary metabolite biosynthesis during in-depth extractive fermentation of Monascus purpureus.  |  Lu, P., et al. 2021. J Biotechnol. 341: 137-145. PMID: 34601020
  12. Effects of triton X-100 pretreatment of lyophilized and frozen-thawed ram sperm on preimplantation embryo developmental competence.  |  Ustuner, B., et al. 2022. Anim Biotechnol. 1-10. PMID: 35200102
  13. Removal of acenaphthene from water by Triton X-100-facilitated biochar-immobilized Pseudomonas aeruginosa.  |  Lu, L., et al. 2018. RSC Adv. 8: 23426-23432. PMID: 35540141
  14. Effect of Green Synthesized Iron Oxide Nanoparticles Using Spinach Extract on Triton X-100-Induced Atherosclerosis in Rats.  |  Obidah Abert, H., et al. 2022. Biochem Res Int. 2022: 9311227. PMID: 36246725
  15. A triton X-100 assisted PMAxx-qPCR assay for rapid assessment of infectious African swine fever virus.  |  Liu, H., et al. 2022. Front Microbiol. 13: 1062544. PMID: 36545208

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Triton X-100, 100 ml

sc-29112
100 ml
$20.00
US: Only available in the US

Triton X-100, 500 ml

sc-29112A
500 ml
$41.00
US: Only available in the US

Hello, Is this product suitable for cell culture?

Asked by: Lorianne
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2023-11-30

Is this material Peroxide and Carbonyl free?

Asked by: Samb
Thank you for your question. Yes, it is peroxide and carbonyl free.
Answered by: Tech Service
Date published: 2019-05-23

May I know what is the pH of the solution? 

Asked by: hhHOR
Unfortunately, pH has not been tested for this product.
Answered by: Technical Support
Date published: 2019-04-12

Buenas tardes.  ¿El precio que manejan es en pesos mexicanos? ¿Cuánto tardaría un envío a FES-Cuautitlán en Cuautitlán izcalli, Estado de México?

Asked by: Alberto 1902
Por favor contacte nuestro distribuidor autorizado en Mexico, Bioselec. Su información se encuentra a través de este enlace: https://www.scbt.com/scbt/resources/distributors Please contact our authorized distributor in Mexico, Bioselec. Their information can be found through this link: https://www.scbt.com/scbt/resources/distributors
Answered by: Technical Support
Date published: 2016-10-14
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Rated 5 out of 5 by from Various publications use Triton XVarious publications use Triton X-100 (sc-29112) in incubation and wash buffers in IHC staining. -SCBT Publication Review
Date published: 2015-07-19
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Triton X-100 is rated 5.0 out of 5 by 1.
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