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- rabbit polyclonal IgG, 200 µg/ml
- epitope mapping at the C-terminus of NF-E2 of mouse origin
- recommended for detection of NF-E2 p45 of mouse, rat and human origin by WB, IP, IF, ELISA and indirect FCM using PE-conjugated goat anti-rabbit IgG: sc-3739; also reactive with additional species, including equine, canine, bovine and porcine
- blocking peptide, sc-291 P
- TransCruz reagent for Gel Supershift and ChIP applications, sc-291 X, 200 µg/0.1 ml
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Ordering InformationProduct Citations
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
NFE2 |
4778 |
12q13.13 |
NM_006163 |
Q16621
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601490 |
| Mouse |
Nfe2 |
18022 |
15 F3 |
|
Q07279
|
N/A |
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NF-E2 Background Information
The nuclear DNA binding protein NF-E2 regulates expression of globulin genes in developing erythroid cells through interaction with upstream AP-1-like recognition sites. More specifically, NF-E2 recognizes a site containing an intact AP-1 binding motif, preceded by a G residue two base pairs upstream. NF-E2 is an obligate heterodimer composed of NF-E2 p45 and NF-E2 p18. NF-E2 p18, also known as NF-E2U or MAFK, is a ubiquitously expressed component that is related to the v-Maf oncogene. It contains a basic-leucine zipper domain that functions in DNA binding and dimerization. In addition, NF-E2 p18 may play a role in erythroid differentiation. The major component of NF-E2 is a polypeptide, designated NF-E2 p45, that belongs to the basic region-leucine zipper family of transcription factors. This subunit of NF-E2 is specifically expressed at low level in hematopoietic progenitor cells and differentiated cells of the erythroid, megakaryocyte and mast cell lineages.
| NF-E2 (C-19) Product Citations |
See how others have used NF-E2 (C-19): sc-291 antibody and or NF-E2 (C-19) antibody conjugates.
23 total citations Loading citations.
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NF-E2 (C-19)
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NF-E2 (C-19): sc-291. Western blot analysis of NF-E2 expression in K-562 nuclear extract.
NF-E2 (C-19): sc-291. Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.
NF-E2 (C-19): sc-291. Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with NF-E2 (C-19), followed by PE-conjugated goat anti-rabbit IgG: sc-3739. Black line histogram represents the isotype control, normal rabbit IgG: sc-3888. NF-E2 siRNA (h): sc-36046. Western blot analysis of NF-E2 expression in non-transfected control (A) and NF-E2 siRNA transfected (B) HeLa cells. Blot probed with NF-E2 (C-19): sc-291. GAPDH (FL-335): sc-25778 used as specificity and loading control. |
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