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home » Signaling Intermediates » NCK Antibody / NCK Antibodies » NCK1/2 (C-19): sc-290

NCK1/2 (C-19) Antibody: sc-290

  |  Datasheet
  • rabbit polyclonal IgG, 200 µg/ml
  • epitope mapping near the C-terminus of NCK1 of human origin
  • recommended for detection of NCK1/2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
  • blocking peptide, sc-290 P
 
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Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   siRNA  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human NCK1 4690 3q22.3 NM_006153 P16333
 600508
Mouse Nck1 17973 9 E3.3 NP_035008
 N/A
 
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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
NCK1/2 (C-19) sc-290 200 µg/ml $279
NCK1/2 (C-19) P sc-290 P
(peptide)
 100 µg/0.5 ml $61
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
NCK1/2 siRNA (h) sc-43959 10 µM $258
NCK1/2 (h)-PR sc-43959-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
NCK1/2 shRNA Plasmid (h) sc-43959-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
NCK1/2 shRNA (h) Lentiviral Particles sc-43959-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
U-937 Cell Lysate sc-2239 500 µg/200 µl $104
A-431 Whole Cell Lysate sc-2201 500 µg/200 µl $104
NCK1 (m): 293T Lysate sc-121953 100µg/200µl $205

NCK1/2 Background Information
The NCK family of SH2/SH3 adaptor proteins consists of two members, NCK1 (NCKå) and NCK2 (NCK∫), which couple tyrosine kinase signaling, including the EGF and PDGF receptor-pathways, to downstream signaling proteins. Specifically, overexpression of Nck1 in NIH/3T3 cells decreases DNA synthesis stimulated by EGF. Furthermore, the SH2 domain of NCK2 inhibits EGF- and PDGF-induced DNA synthesis. The SH3 domain of NCK binds a proline-rich domain on PAK, a known actin cytoskeleton regulator. The NCK protein thus mediates the interaction between PAK and RAC. The NCK2 protein binds human PDGFR-b (Tyr-1009); overexpression of Nck2 inhibits PDGF-induced membrane ruffling and lamellipod formation. Various growth factor receptors, cell surface antigens and adhesion molecules phosphorylate mammalian NCK1 and NCK2. The human NCK1 and NCK2 genes map to chromosomes 3q21 and 2q12, respectively.

NCK1/2 (C-19) Product Citations
See how others have used NCK1/2 (C-19): sc-290 antibody and or NCK1/2 (C-19) antibody conjugates.


8 total citations
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NCK1/2 (C-19)
Click on image to enlarge
sc-290
NCK1/2 (C-19): sc-290. Western blot analysis of NCK1/2 expression in U-937 (A) and A-431 (B) whole cell lysates.
sc-290
NCK1/2 (C-19): sc-290. Western blot analysis of NCK1 expression in non-transfected: sc-117752 (A) and mouse NCK1 transfected: sc-121953 (B) 293T whole cell lysates.
sc-290
NCK1/2 (C-19): sc-290. Western blot analysis of NCK1 expression in non-transfected: sc-117752 (A) and human NCK1 transfected: sc-177604 (B) 293T whole cell lysates and mouse testis tissue extract (C).
sc-290
NCK1/2 (C-19): sc-290. Immunoperoxidase staining of formalin fixed, paraffin-embedded human skin tissue showing cytoplasmic and nuclear staining of epidermal cells.
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