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eIF3 p110 Antibody (H-220): sc-28858

 |  Datasheet

(Based on data analysis)

  • eIF3 p110 Antibody (H-220) is a rabbit polyclonal IgG provided at 200 µg/ml
  • epitope corresponding to amino acids 611-761 mapping within an internal region of eIF3 p110 of human origin
  • recommended for detection of eIF3 p110 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine, canine and bovine
  • See 2 citations for eIF3 p110 Antibody (H-220)
 
Full-length eIF3 p110 protein sequence with immunogen highlighted:
MSRFFTTGSDSESESSLSGEELVTKPVGGNYGKQPLLLSEDEEDTKRVVRSAKDKRFEELTNLIRTIRNAMKIRDVTKCLEEFELLGKAYGKAKSIVDKEGVPRFYIRILADLEDYLNELWEDKEGKKKMNKNNAKALSTLRQKIRKYNRDFESHITSYKQNPEQSADEDAEKNEEDSEGSSDEDEDEDGVSAATFLKKKSEAPSGESRKFLKKMDDEDEDSEDSEDDEDWDTGSTSSDSDSEEEEGKQTALASRFLKKAPTTDEDKKAAEKKREDKAKKKHDRKSKRLDEEEEDNEGGEWERVRGGVPLVKEKPKMFAKGTEITHAVVIKKLNEILQARGKKGTDRAAQIELLQLLVQIAAENNLGEGVIVKIKFNIIASLYDYNPNLATYMKPEMWGKCLDCINELMDILFANPNIFVGENILEESENLHNADQPLRVRGCILTLVERMDEEFTKIMQNTDPHSQEYVEHLKDEAQVCAIIERVQRYLEEKGTTEEVCRIYLLRILHTYYKFDYKAHQRQLTPPEGSSKSEQDQAENEGEDSAVLMERLCKYIYAKDRTDRIRTCAILCHIYHHALHSRWYQARDLMLMSHLQDNIQHADPPVQILYNRTMVQLGICAFRQGLTKDAHNALLDIQSSGRAKELLGQGLLLRSLQERNQEQEKVERRRQVPFHLHINLELLECVYLVSAMLLEIPYMAAHESDARRRMISKQFHHQLRVGERQPLLGPPESMREHVVAASKAMKMGDWKTCHSFIINEKMNGKVWDLFPEADKVRTMLVRKIQEESLRTYLFTYSSVYDSISMETLSDMFELDLPTVHSIISKMIINEELMASLDQPTQTVVMHRTEPTAQQNLALQLAEKLGSLVENNERVFDHKQGTYGGYFRDQKDGYRKNEGYMRRGGYRQQQSQTAY

See additional eIF Antibodies including eIF, eIF1, eIF1/1B, eIF1AD, eIF1AX, eIF1AY, eIF2 alpha, eIF2 beta, eIF2B alpha, eIF2B beta, eIF2B delta, eIF2B epsilon, eIF2B gamma, eIF2C, eIF2D, eIF3 alpha, eIF3 beta, eIF3 delta, eIF3 epsilon, eIF3 eta, eIF3 gamma, eIF3 theta, eIF3 zeta, eIF3e, eIF3K, eIF3M, eIF4A, eIF4AI, eIF4AII, eIF4AIII, eIF4B, eIF4E, eIF4G, eIF4GII, eIF4H, eIF5, eIF5A, eIF5B, eIF6, eIF2C1, eIF2C2, eIF2C3, eIF2S3, eIF2C4, eIF2C5, EIF3S6IP, eIF4E2, eIF4E3 and eIF3 p110.

Ordering InformationProduct CitationsGene Info
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   siRNA  
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
eIF3 p110 Antibody (H-220) sc-28858 200 µg/ml $279
 CRISPR/Cas9 KO and HDR Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
eIF3 p110 CRISPR/Cas9 KO Plasmid (m) sc-425099 20 µg $345
eIF3 p110 HDR Plasmid (m) sc-425099-HDR 20 µg $385
eIF3 p110 CRISPR/Cas9 KO Plasmid (h) sc-417125 20 µg $345
eIF3 p110 HDR Plasmid (h) sc-417125-HDR 20 µg $385
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
eIF3 p110 siRNA (h) sc-40545 10 µM $258
eIF3 p110 siRNA (m) sc-40546 10 µM $258
eIF3 p110 (h)-PR sc-40545-PR 10 µM, 20 µl $23
eIF3 p110 (m)-PR sc-40546-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
eIF3 p110 shRNA Plasmid (h) sc-40545-SH 20 µg $520
eIF3 p110 shRNA Plasmid (m) sc-40546-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
eIF3 p110 shRNA (h) Lentiviral Particles sc-40545-V 200 µl $625
eIF3 p110 shRNA (m) Lentiviral Particles sc-40546-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
F9 Cell Lysate sc-2245 500 µg/200 µl $104
JAR Cell Lysate sc-2276 500 µg/200 µl $104
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104
MIA PaCa-2 Cell Lysate sc-2285 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human EIF3C 8663 16p11.2 NM_001037808, NM_003752 Q99613
603916
Human EIF3CL 728689 16p11.2 XM_001132482, XM_001132505, XM_001132506, XM_001132509, XM_001132522 NP_001093131
Mouse Eif3c 56347 7 F3 Q8R1B4
N/A
 


Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex containing methionyl initiator tRNA (Met-tRNAiMet), which is base paired at the initiation codon (AUG, GUG) in eligible transcripts (1,2). Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation (1-3). Eukaryotic initiation factor-3 (eIF3) is the largest family of eIFs and consists of at least 10 unique subunits (p170, p116, p110, p66, p48, p47, p44, p40, p36, and p35) in mammals (3,4). eIF3 subunit-9 (eIF3-h, eIF3-p116, p116, eIF3-S9, PRT1) is a 873 amino acid component of the eIF3 multi-subunit complex that is involved in ribosomal 48S complex formation (3,5). Association of the eIF3 complex with the 40S ribosomal subunit stabilizes eIF2-GTP-Met-tRNAiMet complex association and mRNA binding, and promotes dissociation of 80S ribosomes into 40S and 60S subunits (6,7).

eIF3 p110 Antibody (H-220) Product Citations
See how others have used eIF3 p110 Antibody (H-220) and or eIF3 p110 Antibody (H-220) conjugates.


2 total citations
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eIF3 p110 Antibody (H-220) Data
Click on image to enlarge
eIF3 p110 (H-220): sc-28858. Western blot analysis of eIF3 p110 expression in 293T (A) and MIA PaCa-2 (B) whole cell lysates.
eIF3 p110 (H-220): sc-28858. Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.
eIF3 p110 (H-220): sc-28858. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
eIF3 p110 (H-220): sc-28858. Western blot analysis of eIF3 p110 expression in JAR (A) and JEG-3 (B) whole cell lysates and mouse placenta tissue extract (C).
eIF3 p110 (H-220): sc-28858. Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast tissue showing cytoplasmic staining of glandular cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
eIF3 p110 (H-220): sc-28858. Western blot analysis of eIF3 p110 expression in non-transfected 293T: sc-117752 (A), mouse eIF3 p110 transfected 293T: sc-126777 (B) and F9 (C) whole cell lysates.
eIF3 p110 (H-220): sc-28858. Western blot analysis of eIF3 p110 expression in non-transfected 293T: sc-117752 (A), human eIF3 p110 transfected 293T: sc-113539 (B) and JAR (C) whole cell lysates.
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