epitope corresponding to amino acids 1-75 mapping at the N-terminus of Hic-5 of human origin
recommended for detection of Hic-5 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine and porcine
TransCruz reagent for Gel Supershift and ChIP applications, sc-28748 X, 200 µg/0.1 ml
Hic-5 Background Information In addition to paxillin, zysin, LPP, Ajuba and TRIP6, hydrogen-peroxide inducible clone 5 (Hic-5) is a member of the LIM family. Hic-5 contains four LIM motifs and seven zinc finger domains. In the cell, Hic-5 localizes to the nuclear matrix and focal adhesion complexes where the LIM domains mediate the interactions of Hic-5 with focal adhesions. Known also as transforming factor beta 1 induced transcript 1, Hic-5 shares extensive homology with the structural protein paxillin, which is involved in the regulation of focal adhesion dynamics. Hic-5 inhibits integrin-mediated cell spreading on Fibronectin by out competing paxillin for focal adhesion kinase and thereby preventing downstream signal transduction. Increased expression of Hic-5 leads to cellular senescence in developing fibroblasts. During myogenesis, expression of Hic-5 blocks differentiation and induces apoptosis of developing myoblasts. The gene encoding human Hic-5 maps to chromosome 16.
Hic-5 (H-75) Product Citations
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Hic-5 (H-75)
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Hic-5 (H-75): sc-28748. Western blot analysis of Hic-5 expression in non-transfected: sc-117752 (A) and human Hic-5 transfected: sc-115206 (B) 293T whole cell lysates.
Hic-5 (H-75): sc-28748. Western blot analysis of Hic-5 expression in non-transfected: sc-117752 (A) and mouse Hic-5 transfected: sc-126953 (B) 293T whole cell lysates.
