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home » Transcription Regulators » TFII Antibody / TFII Antibodies » TFIIE-α (H-300): sc-28715

TFIIE-α (H-300) Antibody: sc-28715

  |  Datasheet
  • rabbit polyclonal IgG, 200 µg/ml
  • epitope corresponding to amino acids 140-439 mapping at the C-terminus of TFIIE-α of human origin
  • recommended for detection of TFIIE-α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
  • TransCruz reagent for Gel Supershift and ChIP applications, sc-28715 X, 200 µg/0.1 ml
 
Additional TFII Antibodies ...
 
Ordering Information
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   Gel Shift   ChIP   siRNA  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human GTF2E1 2960 3q13.33 NM_005513 P29083
 189962
Mouse Gtf2e1 74197 16 B3 Q9D0D5
 N/A
 
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
TFIIE-α (H-300) sc-28715 200 µg/ml $279
TFIIE-α (H-300) X sc-28715 X 200 µg/0.1 ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
TFIIE-α siRNA (h) sc-36651 10 µM $258
TFIIE-α siRNA (m) sc-36652 10 µM $258
TFIIE-α (h)-PR sc-36651-PR 10 µM $23
TFIIE-α (m)-PR sc-36652-PR 10 µM $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
TFIIE-α shRNA Plasmid (h) sc-36651-SH 20 µg $520
TFIIE-α shRNA Plasmid (m) sc-36652-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
TFIIE-α shRNA (h) Lentiviral Particles sc-36651-V 200 µl $625
TFIIE-α shRNA (m) Lentiviral Particles sc-36652-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
NIH/3T3 nuclear extract sc-2138 1000 µg $143
3611-RF nuclear extract sc-2143 1000 µg $143
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104

TFIIE-α Background Information
In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. Human TFIIE consists of two subunits, a and b. The structure of TFIIE appears to be a heterotetramer (å2∫2); both subunits are required for optimal basal-level transcription.

TFIIE-α (H-300)
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sc-28715
TFIIE-α (H-300): sc-28715. Western blot analysis of TFIIE-α expression in NIH/3T3 (A) and Rat 2 (B) whole cell lysates and 3611-RF nuclear extract (C).
sc-28715
TFIIE-α (H-300): sc-28715. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
sc-28715
TFIIE-α (H-300): sc-28715. Immunoperoxidase staining of formalin fixed, paraffin-embedded human oral mucosa tissue showing nuclear staining of surface epithelial cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
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