epitope corresponding to amino acids 91-190 mapping within an internal region of USF-2 of human origin
recommended for detection of USF-2 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including canine, bovine and porcine
TransCruz reagent for Gel Supershift and ChIP applications, sc-28690 X, 200 µg/0.1 ml
USF-2 Background Information The ubiquitously expressed cellular upstream stimulatory factor (USF) consists of USF-1 and USF-2 polypeptides which independently exhibit site-specific DNA binding and are members of the c-Myc-related family of regulatory factors containing helix-loop-helix domains. USF also contains a leucine repeat that is required for efficient DNA binding. USF was originally identified as an upstream stimulatory factor that binds the core sequence CACGTG in the adenovirus late promoter. These findings, together with the demonstration of cooperative interaction between USF and the initiator-binding protein, TFII-I , raises the possibility of a more general involvement of USF in transcriptional regulation. While expression of both USF-1 and USF-2 species is ubiquitous, different ratios of USF homo- and heterodimers are found in different cell types.
