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home » Transcription Regulators » Suppressor of Ty Antibody / Suppressor of Ty Antibodies » SPT5 (H-300): sc-28678

SPT5 (H-300) Antibody: sc-28678

  |  Datasheet
  • rabbit polyclonal IgG, 200 µg/ml
  • epitope corresponding to amino acids 61-360 mapping near the N-terminus of SPT5 of human origin
  • recommended for detection of SPT5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine, canine, porcine and avian
  • TransCruz reagent for Gel Supershift and ChIP applications, sc-28678 X, 200 µg/0.1 ml
 
Additional Suppressor of Ty Antibodies ...
 
Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   Gel Shift   ChIP   siRNA  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human SUPT5H 6829 19q13.2 NM_003169 O00267
 602102
Mouse Supt5h 20924 7 A3 O55201
 N/A
 
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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
SPT5 (H-300) sc-28678 200 µg/ml $279
SPT5 (H-300) sc-28678 X 200 µg/0.1 ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
SPT5 siRNA (h) sc-38440 10 µM $258
SPT5 siRNA (m) sc-38441 10 µM $258
SPT5 siRNA (h) sc-38440-PR 10 µM $23
SPT5 (m)-PR sc-38441-PR 10 µM $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
SPT5 shRNA Plasmid (h) sc-38440-SH 20 µg $520
SPT5 shRNA Plasmid (m) sc-38441-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
SPT5 shRNA (h) Lentiviral Particles sc-38440-V 200 µl $625
SPT5 shRNA (m) Lentiviral Particles sc-38441-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
CCRF-CEM nuclear extract sc-2146 1000 µg $143
DU 145 nuclear extract sc-24960 1000 µg $143
HeLa nuclear extract sc-2120 1000 µg $143
HeLa Whole Cell Lysate sc-2200 500 µg/200 µl $104

SPT5 Background Information
SPT4 (also designated Suppressor of Ty4 and p14) and SPT5 (also designated DSIF p160) are highly conserved proteins from yeast to humans (1). Nuclear SPT4 and SPT5 are involved in both DRB (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole)-mediated transcriptional inhibition as well as the activation of transcriptional elongation by the HIV-1 protein Tat. SPT4 binds SPT5 to form the DSIF (DRB-sensitivity-inducing factor) complex, which binds RNA polymerase II and directly regulates elongation. However, SPT5 protein in mitotic HeLa cells migrates more slowly on SDS-PAGE than does SPT5 isolated from interphase cells, as a result of enhanced SPT5 phosphorylation. The C-terminal CTR1 domain of SPT5 is the substrate for P-TEFb phosphorylation, which is critical for SPT5 function as a regulator of transcriptional elongation.

SPT5 (H-300) Product Citations
See how others have used SPT5 (H-300): sc-28678 antibody and or SPT5 (H-300) antibody conjugates.


6 total citations
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SPT5 (H-300)
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sc-28678
SPT5 (H-300): sc-28678. Western blot analysis of SPT5 expression in CCRF-CEM (A) and DU 145 (B) nuclear extracts.
sc-28678
SPT5 (H-300): sc-28678. Western blot analysis of SPT5 expression in non-transfected: sc-117752 (A) and mouse SPT5 transfected: sc-127585 (B) 293T whole cell lysates and DU 145 nuclear extract (C).
sc-28678
SPT5 (H-300): sc-28678. Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear staining of cells in seminiferous ducts.
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