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home » Transcription Regulators » EF Antibody / EF Antibodies » EF-1α1 (H-300): sc-28578

EF-1α1 (H-300) Antibody: sc-28578

  |  Datasheet
  • rabbit polyclonal IgG, 200 µg/ml
  • epitope corresponding to amino acids 163-462 mapping at the C-terminus of EF-1α1 of human origin
  • recommended for detection of EF-Tu and EF1α2 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
 
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Ordering InformationProduct Citations
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   siRNA  
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human EEF1A1 1915 6q13 NM_001402 P68104
 130590
Human EEF1A2 1917 20q13.33 NM_001958 Q05639
 602959
Mouse Eef1a1 13627 9 E1 P10126
 N/A
Mouse Eef1a2 13628 2 H4 P62631
 N/A
 
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 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
EF-1α1 (H-300) sc-28578 200 µg/ml $279
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
EF-1 α1 siRNA (h) sc-77231 10 µM $258
EF-1 α1 siRNA (m) sc-77232 10 µM $258
EF-1 α1 (h)-PR sc-77231-PR 10 µM $23
EF-1 α1 (m)-PR sc-77232-PR 10 µM $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
EF-1 α1 shRNA Plasmid (h) sc-77231-SH 20 µg $520
EF-1 α1 shRNA Plasmid (m) sc-77232-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
EF-1 α1 shRNA (h) Lentiviral Particles sc-77231-V 200 µl $625
EF-1 α1 shRNA (m) Lentiviral Particles sc-77232-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
EF-1 α1 (h10): 293T Lysate sc-117345 100µg/200µl $205
EF-1 α1 (m3): 293T Lysate sc-119927 100µg/200µl $205
EF-1 α1 (h5): 293 Lysate sc-112341 100µg/200µl $205
EF-1 α1 (h6): 293 Lysate sc-112930 100µg/200µl $205
EF-1 α1 (h7): 293 Lysate sc-112983 100µg/200µl $205

EF-1α1 Background Information
Two elongation factors, mitochondrial EF-1 å1 and EF-2, participate in the elongation phase during protein biosynthesis on the ribosome, and their functional cycles depend on GTP binding and hydrolysis. EF-1 å1 (also designated EF-Tu) and EF-2 are multidomain GTPases with essential functions in translation, and they both bind to the same site on the ribosome, where their low intrinsic GTPase activities are strongly stimulated. mitochondrial EF-1 å1 plays a central role in the fast and accurate delivery of aminoacyl-tRNAs to the translating ribosome. In addition, EF-1 å1 protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anticodon on tRNA can be achieved. EF-2 supports the translocation of tRNAs and of mRNAs on the ribosome so that a new codon can be exposed for decoding.

EF-1α1 (H-300) Product Citations
See how others have used EF-1α1 (H-300): sc-28578 antibody and or EF-1α1 (H-300) antibody conjugates.


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EF-1α1 (H-300)
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sc-28578
EF-1 α1(H-300): sc-28578. Western blot analysis of EF-1 α1 expression in HeLa (A), MOLT-4 (B), A549 (C), JAR (D) and Hep G2 (E) whole cell lysates.
sc-28578
EF-1 α1 (H-300): sc-28578. Western blot analysis of EF-1 α1 expression in non-transfected 293: sc-110760 (A), human EF-1 α1 transfected 293: sc-112930 (B) and HeLa (C) whole cell lysates.
sc-28578
EF-1 α1 (H-300): sc-28578. Western blot analysis of EF-1 α1 expression in non-transfected 293: sc-110760 (A), human EF-1 α1 transfected 293: sc-112983 (B) and HeLa (C) whole cell lysates.
sc-28578
EF-1 α1 (H-300): sc-28578. Western blot analysis of EF-1 a1 expression in non-transfected 293: sc-110760 (A), human EF-1 α1 transfected 293: sc-112341 (B) and Hep G2 (C) whole cell lysates.
sc-28578
EF-1 α1 (H-300): sc-28578. Western blot analysis of EF-1 α1 expression in non-transfected 293T: sc-117752 (A), human EF-1 α1 transfected 293T: sc-113570 (B) and U-87 MG (C) whole cell lysates.
sc-28578
EF-1 α1 (H-300): sc-28578. Western blot analysis of EF-1 α1 expression in non-transfected 293T: sc-117752 (A), mouse EF-1 α1 transfected 293T: sc-119925 (B) and HeLa (C) whole cell lysates.
sc-28578
EF-1 α1 (H-300): sc-28578. Western blot analysis of EF-1 α1 expression in non-transfected: sc-117752 (A) and human EF-1 α1 transfected: sc-117370 (B) 293T whole cell lysates.
sc-28578
EF-1 α1 (H-300): sc-28578. Western blot analysis of EF-1 α1 expression in non-transfected 293T: sc-117752 (A), mouse EF-1 α1 transfected 293T: sc-119928 (B) and Hep G2 (C) whole cell lysates.
sc-28578
EF-1 α1 (H-300): sc-28578. Western blot analysis of EF-1 α1 expression in non-transfected 293T: sc-117752 (A), mouse EF-1 α1 transfected 293T: sc-119927 (B) and HeLa (C) whole cell lysates.
sc-28578
EF-1 α1 (H-300): sc-28578. Western blot analysis of EF-1 α1 expression in non-transfected: sc-117752 (A) and human EF-1 α1 transfected: sc-117345 (B) 293T whole cell lysates.
sc-28578
EF-1 α1 (H-300): sc-28578. Western blot analysis of EF-1 α1 expression in HL-60 whole cell lysate.
sc-28578
EF-1 α1 (H-300): sc-28578. Western blot analysis of EF-1 α1 expression in non-transfected 293T: sc-117752 (A), mouse EF-1 α1 transfected 293T: sc-119931 (B) and HL-60 (C) whole cell lysates.
sc-28578
EF-1 a1 (H-300): sc-28578. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
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