p-Lck (Tyr 192/Ser 194)-R Antibody: sc-28445-R

Lck Background Information
The Src gene family of membrane-associated protein tyrosine kinases include c-Src, c-Yes, Fyn, Lck, Hck, Lyn, Blk and c-Fgr (1). The human Lck gene encodes a lymphocyte-specific tyrosine kinase designated pp56 Lck. Rearrangement and overexpression of the Lck gene occurs in select murine lymphomas (2,3). Human lymphomas and neuroblastomas frequently exhibit chromosomal abnormalities within a site in the genome that contains the Lck gene (4). Resting T cells contain high levels of the Lck protein and mRNA, both of which decline upon activation of T cells (5,6). Lck expression may contribute to the pathogenesis of some types of neoplastic disease (5,6). Csk phosphorylates Lck on Tyr 505 (7,9). This phosphorylation event supresses Lck catalytic activity under certain conditions (7,8). The phosphorylation of Lck at Tyr 394 stimulates Lck activity regardless of Tyr 505 phosphorylation status (8, 9). In response to T cell antigen receptor binding and subsequent signaling, Lck undergoes phosphorylation on residues that include Tyr-192, Ser-59 and Ser-194.