Welcome to Santa Cruz Biotechnology!

PLZF Antibody (D-9): sc-28319

 |  Datasheet

(Based on data analysis)

  • PLZF Antibody (D-9) is a mouse monoclonal IgG1 provided at 200 µg/ml
  • raised against amino acids 101-400 of PLZF of human origin
  • recommended for detection of PLZF of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • See 24 citations for PLZF Antibody (D-9)
 
Full-length PLZF protein sequence with immunogen highlighted:
MDLTKMGMIQLQNPSHPTGLLCKANQMRLAGTLCDVVIMVDSQEFHAHRTVLACTSKMFEILFHRNSQHYTLDFLSPKTFQQILEYAYTATLQAKAEDLDDLLYAAEILEIEYLEEQCLKMLETIQASDDNDTEATMADGGAEEEEDRKARYLKNIFISKHSSEESGYASVAGQSLPGPMVDQSPSVSTSFGLSAMSPTKAAVDSLMTIGQSLLQGTLQPPAGPEEPTLAGGGRHPGVAEVKTEMMQVDEVPSQDSPGAAESSISGGMGDKVEERGKEGPGTPTRSSVITSARELHYGREESAEQVPPPAEAGQAPTGRPEHPAPPPEKHLGIYSVLPNHKADAVLSMPSSVTSGLHVQPALAVSMDFSTYGGLLPQGFIQRELFSKLGELAVGMKSESRTIGEQCSVCGVELPDNEAVEQHRKLHSGMKTYGCELCGKRFLDSLRLRMHLLAHSAGAKAFVCDQCGAQFSKEDALETHRQTHTGTDMAVFCLLCGKRFQAQSALQQHMEVHAGVRSYICSECNRTFPSHTALKRHLRSHTGDHPYECEFCGSCFRDESTLKSHKRIHTGEKPYECNGCGKKFSLKHQLETHYRVHTGEKPFECKLCHQRSRDYSAMIKHLRTHNGASPYQCTICTEYCPSLSSMQKHMKGHKPEEIPPDWRIEKTYLYLCYV

See additional PLZF Antibodies.

Ordering InformationProduct CitationsGene Info
Recommended Support Products:
(click button of application of choice)
WB   IP   IF   IHC(P)   siRNA  
Set Currency

 Ordering Information
Product NameCatalog #UnitPriceQtyAddFavorites
PLZF Antibody (D-9) sc-28319 200 µg/ml $279
 CRISPR/Cas9 KO and HDR Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
PLZF CRISPR/Cas9 KO Plasmid (h) sc-400354 20 µg $345
PLZF HDR Plasmid (h) sc-400354-HDR 20 µg $385
PLZF CRISPR/Cas9 KO Plasmid (m) sc-433445 20 µg $345
PLZF HDR Plasmid (m) sc-433445-HDR 20 µg $385
 siRNA Gene Silencers (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
PLZF siRNA (h) sc-37149 10 µM $258
PLZF siRNA (m) sc-37150 10 µM $258
PLZF siRNA (r) sc-156168 10 µM $258
PLZF (h)-PR sc-37149-PR 10 µM, 20 µl $23
PLZF (m)-PR sc-37150-PR 10 µM, 20 µl $23
 shRNA Plasmids (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
PLZF shRNA Plasmid (h) sc-37149-SH 20 µg $520
PLZF shRNA Plasmid (m) sc-37150-SH 20 µg $520
 shRNA Lentiviral Particles (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
PLZF shRNA (h) Lentiviral Particles sc-37149-V 200 µl $625
PLZF shRNA (m) Lentiviral Particles sc-37150-V 200 µl $625
 WB Positive Control Cell Lysates (click product name for more information)
Product NameCatalog #UnitPriceQtyAddFavorites
HEL 92.1.7 Cell Lysate sc-2270 500 µg/200 µl $104
TF-1 Cell Lysate sc-2412 500 µg/200 µl $104
 
Species Gene Name Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
Human ZBTB16 7704 11q23.2 NM_001018011, NM_006006 Q05516
176797
Mouse Zbtb16 235320 9 A5.3 NP_001028496
N/A
 


Hypermethylated in cancer (HIC-1) was originally identified as a target of p53-induced gene expression. HIC-1 is deleted in the genetic disorder Miller-Dieker syndrome (MDS). The expression of HIC-1 is also frequently suppressed in leu-kemia and other various cancers due to the hypermethylation of specific DNA regions and the resulting transcriptional silencing. These and other studies indicate that HIC-1 acts as a putative tumor suppressor protein that mediates transcriptional repression. HIC-1 is ubiquitously expressed in adult tissues and its structure is defined by five zinc fingers and an N-terminal broad-complex POZ (or BTB) domain. The BTB/POZ domain mediates homomeric and heteromeric POZ-POZ interactions and is common to transcriptional regulators involved in chromatin modeling. In several BTB/POZ containing proteins, including BCL-6 and the promyelocytic leukemia zinc finger (PLZF) oncoprotein, this domain interacts with the SMRT/N-CoR-mSin3A HDAC complex and is directly involved in repressing and silencing gene transcription. When this domain is deleted, as with the oncogenic PLZF-RAR chimera of promyelocytic leukemias, this transcriptional repression is attenuated. Conversely, HIC-1 does not interact with components of the HDAC complex, suggesting that HIC-1-induced transcriptional repression is unassociated with the POZ/BTB domain.

PLZF Antibody (D-9) Product Citations
See how others have used PLZF Antibody (D-9) and or PLZF Antibody (D-9) conjugates.


24 total citations
Loading citations.
 
PLZF Antibody (D-9) Data
Click on image to enlarge
PLZF (D-9): sc-28319. Immunoperoxidase staining of formalin fixed, paraffin-embedded human thyroid gland tissue showing nuclear staining of glandular cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
PLZF (D-9): sc-28319. Western blot analysis of PLZF expression in HEL 92.1.7 whole cell lysate.
PLZF (D-9): sc-28319. Western blot analysis of PLZF expression in non-transfected: sc-117752 (A) and human PLZF transfected: sc-113938 (B) 293T whole cell lysates.
PLZF (D-9): sc-28319. Western blot analysis of PLZF expression in non-transfected: sc-117752 (A) and human PLZF transfected: sc-114505 (B) 293T whole cell lysates.
PLZF (D-9): sc-28319. Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing nuclear staining of subset of glandular cells.
Download