GABP-α Antibody (G-1): sc-28312

GABP-α Antibody (G-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects GABP-alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-GABP-alpha antibody (G-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. GABP-alpha protein, a crucial component of the GA-binding protein transcription factor complex, plays a significant role in regulating gene expression by binding to specific DNA sequences. GABP-alpha protein is primarily located in the nucleus, where GABP-alpha forms a heterodimer with the GABPβ subunit, which exists in two splice variants, b1 and b2, differing in their C-termini. Nuclear localization of GABP-alpha is essential for modulating transcriptional activity, as GABP-alpha coordinates the expression of genes involved in critical cellular processes, including mitochondrial function and stress responses. GABPαβ tetrameric complex stimulates transcription of the adenovirus E4 gene, activates BRCA1 expression in human breast cell lines, enhances Tat-mediated transcription in certain cell types, and regulates the transcription of the rpL32 gene, highlighting GABP-alpha′s importance in both normal physiology and disease states.

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GABP-α Antibody (G-1) References:

1. Synergistic transcriptional activation by hGABP and select members of the activation transcription factor/cAMP response element-binding protein family.  |  Sawada, J., et al. 1999. J Biol Chem. 274: 35475-82. PMID: 10585419
2. The alpha and beta subunits of the GA-binding protein form a stable heterodimer in solution. Revised model of heterotetrameric complex assembly.  |  Chinenov, Y., et al. 2000. J Biol Chem. 275: 7749-56. PMID: 10713087
3. Depolarizing stimulation upregulates GA-binding protein in neurons: a transcription factor involved in the bigenomic expression of cytochrome oxidase subunits.  |  Zhang, C. and Wong-Riley, MT. 2000. Eur J Neurosci. 12: 1013-23. PMID: 10762332
4. GA-binding protein alpha/beta is a critical regulator of the BRCA1 promoter.  |  Atlas, E., et al. 2000. Oncogene. 19: 1933-40. PMID: 10773883
5. The ETS transcription factor GABPalpha is essential for early embryogenesis.  |  Ristevski, S., et al. 2004. Mol Cell Biol. 24: 5844-9. PMID: 15199140
6. Identification of functional elements in the murine Gabp alpha/ATP synthase coupling factor 6 bi-directional promoter.  |  Patton, J., et al. 2006. Gene. 369: 35-44. PMID: 16309857
7. GABPalpha regulates Oct-3/4 expression in mouse embryonic stem cells.  |  Kinoshita, K., et al. 2007. Biochem Biophys Res Commun. 353: 686-91. PMID: 17194449
8. Targeting of the ETS factor GABPalpha disrupts neuromuscular junction synaptic function.  |  O'Leary, DA., et al. 2007. Mol Cell Biol. 27: 3470-80. PMID: 17325042
9. Neuregulin-1 induces acetylcholine receptor transcription in the absence of GABPalpha phosphorylation.  |  Herndon, CA. and Fromm, L. 2008. J Neurosci Res. 86: 982-91. PMID: 17975830
10. Critical requirement of GABPalpha for normal T cell development.  |  Yu, S., et al. 2010. J Biol Chem. 285: 10179-88. PMID: 20139079
11. Functional interactions of transcription factor human GA-binding protein subunits.  |  Suzuki, F., et al. 1998. J Biol Chem. 273: 29302-8. PMID: 9792629
12. Evolution of the human immunodeficiency virus type 1 long terminal repeat promoter by conversion of an NF-kappaB enhancer element into a GABP binding site.  |  Verhoef, K., et al. 1999. J Virol. 73: 1331-40. PMID: 9882338

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-GABP-α Antibody (G-1) has 19 citations in a variety of scientific publications.

Primary antibodies like Anti-GABP-α Antibody (G-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

GABP-α Antibody (G-1) Specifications

Alternate Product Name: GABPA

Product Clone Name: G-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-180

Epitope Target: GABP-α

Epitope Species: human

Product Reactivity/Detection: GABP-α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for GABP-α Antibody (G-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent