RFC4 (E-12) Antibody: sc-28300

RFC4 Background Information
Replication factor C (RFC) is an essential DNA polymerase accessory protein that is required for numerous aspects of DNA metabolism including DNA replication, DNA repair, and telomere metabolism. RFC is a heteropentameric complex that recognizes a primer on a template DNA, binds to a primer terminus, and loads proliferating cell nuclear antigen (PCNA) onto DNA at primer-template junctions in an ATP-dependent reaction. All five of the RFC subunits share a set of related sequences (RFC boxes) that include nucleotide-binding consensus sequences. Four of the five RFC genes (RFC1, RFC2, RFC3, and RFC4) have consensus ATP-binding motifs. The small RFC proteins, RFC2, RFC3, RFC4 and RFC5, interact with Rad24, whereas the RFC1 subunit does not. Specifically, RFC4 plays a role in checkpoint regulation. RFC4 is a component of BASC (for BRCA1-associated genome surveillance complex) which serves as a sensor for abnormal DNA structures and/or as a regulator of the postreplication repair process. The human RFC4 gene maps to chromosome 3q27.3 and encodes the RFC4 subunit.