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- mouse monoclonal IgG1; 200 µg/ml
- raised against a short amino acid sequence containing phosphorylated Ser 721
- recommended for detection of Ser 721 phosphorylated Stat4 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
- TransCruz reagent for Gel Supershift and ChIP applications, sc-28296 X, 200 µg/0.1 ml
- blocking peptide, sc-28296 P
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Ordering InformationProduct Citations
Recommended Support Products:
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| Species |
Gene Name |
Gene ID |
Chromosome Location |
Isoform (mRNA) Accession # |
Protein Accession # |
OMIM™ Number |
| Human |
STAT4 |
6775 |
2q32.3 |
NM_003151 |
Q14765
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600558 |
| Mouse |
Stat4 |
20849 |
1 C1.1 |
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P42228
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N/A |
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Stat4 Background Information
Membrane receptor signaling by various ligands, including interferons and growth hormones, induces activation of Jak kinases, which then leads to tyrosine phosphorylation of the Stat transcription factors. Upon activation by tyrosine phosphorylation, Stat proteins dimerize, translocate to the nucleus and bind to specific regulatory elements that control gene expression. Stat4 is most highly expressed in testis and myeloid cells and is an important element in mediating IL-12 signals. IL-12 induces sustained activation and nuclear translocation of Stat4, a process coupled to both tyrosine and serine phosphorylation of Stat4. Phosphorylation of Ser 721 of Stat4 is MEK-, ERK- and JNK-independent and p38-dependent, and is necessary for the transcriptional activity of Stat4.
| p-Stat4 (E-2) Product Citations |
See how others have used p-Stat4 (E-2): sc-28296 antibody and or p-Stat4 (E-2) antibody conjugates.
2 total citations Loading citations.
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p-Stat4 (E-2)
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p-Stat4 (E-2): sc-28296. Western blot analysis of Stat4 phosphorylation in untreated (A) and PMA + LPS-treated RAW 264.7 (B) whole cell lysates.
p-Stat4 (E-2): sc-28296. Immunofluorescence staining of methanol-fixed IFN-γ-treated HeLa cells showing nuclear localization.
p-Stat4 (E-2): sc-28296. Western blot analysis of Stat4 phosphorylation in untreated (A) and PMA + LPS-treated RAW 264.7 (B) whole cell lysates.
p-Stat4 (E-2): sc-28296. Western blot analysis of Stat4 phosphorylation in untreated (A) and lambda protein phosphatase treated (B) HEK293 whole cell lysates.
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