epitope corresponding to amino acids 324-623 mapping at the C-terminus of Chfr of human origin
recommended for detection of Chfr of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
Chfr Background Information The forkhead-associated (FHA) domain was initially identified in transcription factors that have forkhead DNA-binding domains and in protein kinases, but many cell-cycle checkpoint proteins, including chfr (checkpoint with forkhead and ring finger domains) contain FHA domains. Chfr defines a checkpoint that delays entry into metaphase in response to mitotic stress. Normal primary cells and tumor cell lines that express wild-type chfr exhibit delayed entry into metaphase when centrosome separation is inhibited by mitotic stress. Additionally, chfr seems to be required for delaying prophase in human cells. The sequence of chfr is similar to that of the fission yeast DMA1, which is involved in a later mitotic checkpoint that delays a cell's exit from mitosis in response to spindle damage.
Chfr (H-300)
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Chfr (H-300): sc-28263. Western blot analysis of Chfr expression in ES-2 whole cell lysate.
