p15/p16 (H-43) Antibody: sc-28260

p15/p16 Background Information
The normal progression of cells through the cell cycle is under the control of the cyclin-dependent protein kinases Cdk4 and Cdk6, which are subject to inhibition by the mitotic inhibitory protein p16. The complexes formed by Cdk4 and the D-type cyclins have been strongly implicated in the control of cell proliferation during the G1 phase. It has been shown that p16 binds to Cdk4 and inhibits the catalytic activity of the Cdk4/cyclin D complex. Moreover, the gene encoding p16 exhibits a high frequency of homozygous deletions and point mutations in established human tumor cell lines. Expression of p15, a member of the p16 family, is upregulated approximately 30-fold in TGF∫-treated human keratinocytes, suggesting that p15 may act as an effector of TGF∫-mediated cell cycle arrest. The gene encoding p15 has been mapped to chromosome 9p21, adjacent to the p16 gene, at a site of frequent chromosomal abnormality in human tumors. It has been suggested that p15 may function as an effector of TGF∫-mediated cell cycle arrest through inhibition of Cdk4 and Cdk6 kinases.