epitope corresponding to amino acids 229-328 mapping at the C-terminus of BUB3 of human origin
recommended for detection of BUB3 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine and porcine
BUB3 Background Information BUB3 (budding uninhibited by benzimidazoles 3 homolog), also known as BUB3L or hBUB3, is a conserved component of the mitotic spindle assembly complex (MCC). It contains five WD repeat domains and forms cell cycle constitutive complexes with BUB1 and BUBR1. BUB3 is essential for the kinetochore localization of BUB1 and BUBR1. As a component of the MCC, BUB3 is involved in the essential spindle checkpoint pathway that operates during early embryogenesis. The spindle checkpoint pathway functions to postpone the initiation of anaphase until chromosomes are properly attached to the spindle. This acts to ensure accurate chromosome segregation. In addition, BUB3 plays a role in regulating the establishment of correct kinetochore-microtubule attachments. BUB3 is also thought to bind Tctex1L (or DYNLT3), a dynein light chain.
BUB3 (H-100)
Click on image to enlarge
BUB3 (H-100): sc-28258. Western blot analysis of BUB3 expression in Jurkat nuclear extract.
BUB3 (H-100): sc-28258. Western blot analysis of BUB3 expression in non-transfected: sc-117752 (A) and mouse BUB3 transfected: sc-126518 (B) 293T whole cell lysates and mouse embryo tissue extract (C).
