epitope corresponding to amino acids 163-462 mapping at the C-terminus of E4BP4 of human origin
recommended for detection of E4BP4 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including bovine and porcine
E4BP4 Background Information E4BP4, also known as NFIL3, functions as a transcriptional repressor and is a member of the basic leucine zipper (bZIP) transcription factor family. E4BP4 binds with high specificity to the E4 ATF, which is a DNA sequence traditionally targeted by the ATF/CREB family of transcription factors. A 65 amino acid segment located in the carboxy-terminus of E4BP4 interacts specifically with the TBP binding protein Dr1. In the suprachiasmatic nucleus, circadian center and liver, E4BP4 competes with PAR proteins for DNA binding via a reciprocating mechanism. The phase expression of E4BP4 correlates with the circadian cycle and represses transcription of genes otherwise activated by PAR transcription regulators. E4BP4 also plays an important role in an IL-3-mediated signaling pathway that is responsible for the survival of B cell progenitors. The gene encoding human E4BP4 maps to chromosome 9q22.
E4BP4 (H-300)
Click on image to enlarge
E4BP4 (H-300): sc-28203. Western blot analysis of E4BP4 expression in rat liver (A) and mouse placenta (B) tissue extracts.
E4BP4 (H-300): sc-28203. Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
E4BP4 (H-300): sc-28203. Western blot analysis of E4BP4 expression in non-transfected: sc-110760 (A) and human E4BP4 transfected: sc-110510 (B) 293 whole cell lysates.
E4BP4 (H-300): sc-28203. Western blot analysis of E4BP4 expression in non-transfected 293T: sc-117752 (A), mouse E4BP4 transfected 293T: sc-119892 (B) and HeLa (C) whole cell lysates.
E4BP4 (H-300): sc-28203. Western blot analysis of E4BP4 expression in 293T (A) and HeLa (B) whole cell lysates.
