Tris Acetate-EDTA buffer, 50X

Tris-Acetate-EDTA (TAE) Buffer is supplied as a 50X concentrate that can be diluted to a 1X solution containing 40 mM Tris-acetate, and 1 mM EDTA, pH ~8.3. Tris Acetate-EDTA buffer is used for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but may become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. May be used at various concentrations to study the mobility of DNA in solution. Since borate in TBE buffer (Tris-Borate-EDTA Buffer, 10X Powder Pack, sc-296651) is a strong inhibitor for many enzymes, TAE buffer is recommended when looking at enzymatic applications for the DNA sample.
Additional forms available:
Tris Acetate-EDTA buffer (sc-296645)
Tris Acetate-EDTA buffer, 10X (sc-296647)

Ready for use following dilution to working concentrations. 50X concentrate can be diluted to a 1X solution containing 40 mM Tris, 20 mM acetate, and 1 mM EDTA, pH ~8.3. May also be added to an acrylamide/bis-acrylamide stock solution for making PAGE gels. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

SDS

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