Agarose, low gelling temperature (CAS 39346-81-1)

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Synonym:	2-Hydroxyethyl agarose
Application:	An agarose with low gelling and melting points for DNA recovery and purification
CAS Number:	39346-81-1

Description

Produces lower gelling and melting points and provides convenient recovery and purification of DNA from low melting agarose gels. Radiolabeled DNA probes can be made directly in remelted agarose using DNA gel slices cut from low-melting agarose gels. Cloning of restriction fragments into plasmids can be conveniently carried out directly in remelted agarose using DNA gel slices cut from low-melting agarose gels. Gels exhibit low background fluorescence using ethidium bromide staining.

Technical Information

Appearance:	White to off-white powder
Storage:	Store at room temperature

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

References

Sambrook, J., et al. Molecular Cloning: A Laboratory Manual 2nd ed., Plainview, NY , (1989), 6. Wieslander, L., A simple method to recover intact high molecular weight RNA and DNA after electrophoretic separation in low gelling temperature agarose gels. Anal. Biochem. 98, 305, (1979) Feinberg, A.P., Vogelstein, B., A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Addendum. Anal. Biochem. 137, 266, (1984); Struhl, K. Biotechniques 3, 452, (1985)

SDS

Datasheet/SDS