epitope corresponding to phosphorylated Ser 166 of MEK kinase-3 of human origin
recommended for detection of Ser 166 phosphorylated MEK kinase-3 of mouse, rat and human origin by WB, IF and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
MEK kinase-3 Background Information Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also called MAP kinase kinase kinases) phosphorylate and activate the MAP kinases, including ERK, JNK and p38. The MEK kinases characterized to date include Raf-1, Raf-B, MOS, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4 and ASK 1 (also designated MEK kinase-5). MEK kinase-1 has been shown to phosphorylate MEK-1 via a Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK pathway. ASK1 activates both MEK-4 and MEK-3/MEK-6 pathways. SGK1 inhibits MEK-3-MKK3/6 signal transduction by phosphorylation of MEK-3 on Ser166 and Ser337.
