Net1 (yE-15) Antibody: sc-27758

Net1 Background Information
The protein phosphatase Cdc14 controls exit from mitosis in budding yeast. Nucleolar protein involved in exit from mitosis (Net1) sequesters Cdc14 protein in the nucleolus prior to anaphase. During anaphase, the regulator of nucleolar silencing and telophase exit (RENT) complex, which contains Net1, dissassembles, allowing Cdc14 to relocate to the nucleus and cytoplasm to catalyze mitotic exit. Another cell cycle protein, cdc5, regulates the release of cdc14 from Net1 by effecting the phosphorylation state of Net1. Mutations of the Net1 gene and alteration of this signaling network affect the maintenance of the mitochondrial genome, chromosomes and plasmids, implying that Net1 occupies a central position in the yeast genetic regulation network.