MBD4 Antibody (D-6): sc-271530

MBD4 Antibody (D-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects MBD4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MBD4 (D-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. MBD4 plays a crucial role in the DNA damage repair process, specifically in the recognition and repair of mismatched base pairs that can arise during DNA replication. This function is vital for maintaining genomic stability and preventing mutations that could lead to diseases such as cancer. MBD4 is known to interact with the mismatch repair protein MLH1, which enhances binding to methylated cytosine residues in mismatched base pairs, thereby facilitating the repair process. Expression of MBD4 is particularly prominent in somatic tissues, where MBD4 contributes to the regulation of gene expression through involvement in DNA methylation pathways. Using anti-MBD4 antibody (D-6) in research can provide insights into the mechanisms of DNA repair and the implications of methylation in gene regulation, which are essential for understanding various biological processes and disease states.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

MBD4 Antibody (D-6) References:

1. Genomic structure and chromosomal mapping of the murine and human Mbd1, Mbd2, Mbd3, and Mbd4 genes.  |  Hendrich, B., et al. 1999. Mamm Genome. 10: 906-12. PMID: 10441743
2. Methylation-mediated transcriptional silencing in euchromatin by methyl-CpG binding protein MBD1 isoforms.  |  Fujita, N., et al. 1999. Mol Cell Biol. 19: 6415-26. PMID: 10454587
3. MBD2 is a transcriptional repressor belonging to the MeCP1 histone deacetylase complex.  |  Ng, HH., et al. 1999. Nat Genet. 23: 58-61. PMID: 10471499
4. The thymine glycosylase MBD4 can bind to the product of deamination at methylated CpG sites.  |  Hendrich, B., et al. 1999. Nature. 401: 301-4. PMID: 10499592
5. Solution structure of the methyl-CpG-binding domain of the methylation-dependent transcriptional repressor MBD1.  |  Ohki, I., et al. 1999. EMBO J. 18: 6653-61. PMID: 10581239
6. DNA methylation inhibits transcription indirectly via a methyl-CpG binding protein.  |  Boyes, J. and Bird, A. 1991. Cell. 64: 1123-34. PMID: 2004419
7. MBD4 and TDG: multifaceted DNA glycosylases with ever expanding biological roles.  |  Sjolund, AB., et al. 2013. Mutat Res. 743-744: 12-25. PMID: 23195996
8. Germline MBD4 deficiency causes a multi-tumor predisposition syndrome.  |  Palles, C., et al. 2022. Am J Hum Genet. 109: 953-960. PMID: 35460607
9. MBD4 loss results in global reactivation of promoters and retroelements with low methylated CpG density.  |  Papin, C., et al. 2023. J Exp Clin Cancer Res. 42: 301. PMID: 37957685
10. Familial uveal melanoma and other tumors in 25 families with monoallelic germline MBD4 variants.  |  Villy, MC., et al. 2024. J Natl Cancer Inst. 116: 580-587. PMID: 38060262
11. Transcriptional repression by the methyl-CpG-binding protein MeCP2 involves a histone deacetylase complex.  |  Nan, X., et al. 1998. Nature. 393: 386-9. PMID: 9620804
12. Identification and characterization of a family of mammalian methyl-CpG binding proteins.  |  Hendrich, B. and Bird, A. 1998. Mol Cell Biol. 18: 6538-47. PMID: 9774669

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MBD4 Antibody (D-6) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-MBD4 Antibody (D-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MBD4 Antibody (D-6) Specifications

Alternate Product Name: MBD4

Product Clone Name: D-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 515-555

Epitope Target: MBD4

Epitope Species: mouse

Product Reactivity/Detection: MBD4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for MBD4 Antibody (D-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin