V-ATPase H Antibody (E-10) | Santa Cruz Biotech

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V-ATPase H Antibody (E-10): sc-271186	\| Datasheet

5 4 (Based on data analysis) 1

V-ATPase H Antibody (E-10) is a mouse monoclonal IgG_2a provided at 200 µg/ml
specific for an epitope mapping between amino acids 10-41 at the N-terminus of V-ATPase H of human origin
recommended for detection of V-ATPase H of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian

See additional V-ATPase Antibodies including V-ATPase A4, V-ATPase A2, V-ATPase B2, V-ATPase C2, V-ATPase D2, V-ATPase G2, V-ATPase A1, V-ATPase alpha 1, V-ATPase B1, V-ATPase C1, V-ATPase D1, V-ATPase G1, V-ATPase, V-ATPase D, V-ATPase E2, V-ATPase F, V-ATPase G3 and V-ATPase H.

Also see V-ATPase Inhibitors for functional analysis of cellular responses to V-ATPase.

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WB IP IF IHC(P)

Ordering Information

Product Name	Catalog #	Unit	Price	Qty	Add
V-ATPase H Antibody (E-10)	sc-271186	200 µg/ml	$279

Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession #	Protein Accession #	OMIM™ Number

Human	ATP6V1H	51606	8q11.23	NM_015941, NM_213619, NM_213620	NP_057025	608861
Mouse	Atp6v1h	108664	1 A1	NM_133826	Q8BVE3	N/A

Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis, and a integral V0 domain, which is responsible for proton translocation, compose V-ATPase (1). Nine subunits (A–H) make up the V1 domain and five subunits (a, d, c, c' and c") make up the V0 domain (1). Like F-ATPase, V-ATPase most likely operates through a rotary mechanism (1). The H subunit of V-ATPase, also designated SDF is comprised of two polypeptides derived from the same gene (2-4). This regulatory subunit plays a critical role in the functional coupling of ATP hydrolysis activity to proton transport in the V-ATPase pump (5).

V-ATPase H Antibody (E-10) Data

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V-ATPase H (E-10): sc-271186. Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.

V-ATPase H (E-10): sc-271186. Western blot analysis of V-ATPase H expression in DU 145 whole cell lysate.

V-ATPase H (E-10): sc-271186. Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic staining of urothelial cells.

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V-ATPase H Antibody (E-10): sc-271186