GATA3 Antibody (HG3-31): sc-268
- GATA3 Antibody (HG3-31) is a mouse monoclonal IgG1 κ GATA3 antibody, cited in 425 publications, provided at 200 µg/ml
- raised against recombinant GATA-3 of human origin
- GATA3 Antibody (HG3-31) is recommended for detection of GATA-3 of mouse, rat, human and avian origin by WB, IP, IF and IHC(P)
- Anti-GATA3 Antibody (HG3-31) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for Gel Supershift and ChIP applications (sc-268 X, 200 µg/0.1 ml)
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of GATA3 (HG3-31): sc-268.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for GATA3 Antibody (HG3-31) for WB and IHC(P) applications. These reagents are now offered in bundles with GATA3 Antibody (HG3-31) (see ordering information below).
GATA-3 Antibody (HG3-31) is a mouse monoclonal IgG1 kappa light chain antibody that detects GATA-3 protein of mouse, rat, human, and avian origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry (IHC). GATA-3 monoclonal antibody (HG3-31) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. GATA-3 plays a crucial role in the development and function of T lymphocytes, acting as a transcription factor that regulates the expression of genes essential for T cell differentiation and function. GATA-3 expression is particularly important in the immune response, as GATA-3 is involved in the development of Th2 cells, which are critical for orchestrating responses to extracellular pathogens and in allergic reactions. GATA-3 is predominantly located in the nucleus of T cells, where GATA-3 binds to specific DNA sequences to modulate gene expression. Nuclear localization is vital for function, as direct influence on the transcription of target genes drives T cell development and function. GATA-3 regulation of these processes underscores significance in both normal immune function and in the pathogenesis of various diseases, including allergies and autoimmune disorders. GATA-3 monoclonal antibody (HG3-31) provides a powerful tool for researchers studying T cell biology and the underlying mechanisms of immune responses.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
GATA3 Antibody (HG3-31) References:
- Human transcription factor GATA-2. Evidence for regulation of preproendothelin-1 gene expression in endothelial cells. | Dorfman, DM., et al. 1992. J Biol Chem. 267: 1279-85. PMID: 1370462
- CTLA-4 regulates allergen response by modulating GATA-3 protein level per cell. | Nasta, F., et al. 2007. Immunology. 121: 62-70. PMID: 17313444
- GATA-3 - not just for Th2 cells anymore. | Ho, IC. and Pai, SY. 2007. Cell Mol Immunol. 4: 15-29. PMID: 17349208
- Gata-3 and mammary cell fate. | Naylor, MJ. and Ormandy, CJ. 2007. Breast Cancer Res. 9: 302. PMID: 17381824
- 4-1BB triggers IL-13 production from T cells to limit the polarized, Th1-mediated inflammation. | Shin, SM., et al. 2007. J Leukoc Biol. 81: 1455-65. PMID: 17389581
- Murine and human T-lymphocyte GATA-3 factors mediate transcription through a cis-regulatory element within the human T-cell receptor delta gene enhancer. | Ko, LJ., et al. 1991. Mol Cell Biol. 11: 2778-84. PMID: 2017177
- GATA-4/5/6, a subfamily of three transcription factors transcribed in developing heart and gut. | Laverriere, AC., et al. 1994. J Biol Chem. 269: 23177-84. PMID: 8083222
- DNA-binding specificities of the GATA transcription factor family. | Ko, LJ. and Engel, JD. 1993. Mol Cell Biol. 13: 4011-22. PMID: 8321208
- The human GATA-6 gene: structure, chromosomal location, and regulation of expression by tissue-specific and mitogen-responsive signals. | Suzuki, E., et al. 1996. Genomics. 38: 283-90. PMID: 8975704
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GATA3 Antibody (HG3-31) | sc-268 | 200 µg/ml | $316.00 | |||
GATA3 Antibody (HG3-31): m-IgG Fc BP-HRP Bundle | sc-528106 | 200 µg Ab; 10 µg BP | $354.00 | |||
GATA3 Antibody (HG3-31): m-IgGκ BP-HRP Bundle | sc-520404 | 200 µg Ab, 40 µg BP | $354.00 | |||
GATA3 Antibody (HG3-31): m-IgG1 BP-HRP Bundle | sc-542762 | 200 µg Ab; 20 µg BP | $354.00 | |||
GATA3 Antibody (HG3-31) X | sc-268 X | 200 µg/0.1 ml | $316.00 | |||
GATA3 Antibody (HG3-31) AC | sc-268 AC | 500 µg/ml, 25% agarose | $416.00 | |||
GATA3 Antibody (HG3-31) HRP | sc-268 HRP | 200 µg/ml | $316.00 | |||
GATA3 Antibody (HG3-31) FITC | sc-268 FITC | 200 µg/ml | $330.00 | |||
GATA3 Antibody (HG3-31) PE | sc-268 PE | 200 µg/ml | $343.00 | |||
GATA3 Antibody (HG3-31) Alexa Fluor® 488 | sc-268 AF488 | 200 µg/ml | $357.00 | |||
GATA3 Antibody (HG3-31) Alexa Fluor® 546 | sc-268 AF546 | 200 µg/ml | $357.00 | |||
GATA3 Antibody (HG3-31) Alexa Fluor® 594 | sc-268 AF594 | 200 µg/ml | $357.00 | |||
GATA3 Antibody (HG3-31) Alexa Fluor® 647 | sc-268 AF647 | 200 µg/ml | $357.00 | |||
GATA3 Antibody (HG3-31) Alexa Fluor® 680 | sc-268 AF680 | 200 µg/ml | $357.00 | |||
GATA3 Antibody (HG3-31) Alexa Fluor® 790 | sc-268 AF790 | 200 µg/ml | $357.00 |