HIF-1α Gel Shift Oligonucleotides: sc-2625

HIF-1α Gel Shift Oligonucleotides are short DNA sequences meticulously synthesized for use in gel shift assays, a crucial technique in molecular biology research aimed at elucidating protein-DNA interactions. The term HIF-1α refers to hypoxia-inducible factor 1-alpha, a key transcription factor involved in cellular responses to hypoxia or low oxygen levels. HIF-1α is a subunit of the HIF-1 heterodimeric transcription factor complex, which regulates the expression of genes involved in adaptation to hypoxic conditions, including angiogenesis, erythropoiesis, glucose metabolism, and cell survival. Under normoxic conditions, HIF-1α is hydroxylated by prolyl hydroxylase enzymes, leading to its degradation via the ubiquitin-proteasome pathway. However, under hypoxic conditions, HIF-1α is stabilized and translocates to the nucleus, where it forms a complex with HIF-1β and other coactivators, binding to specific DNA sequences, known as hypoxia response elements (HREs), within the promoters of target genes, thereby modulating their transcriptional activity. By utilizing HIF-1α Gel Shift Oligonucleotides in gel shift assays, researchers can investigate the binding kinetics, specificity, and affinity of HIF-1α to its target DNA sequences under various experimental conditions. This technique facilitates the elucidation of the molecular mechanisms underlying HIF-1α-mediated gene regulation and provides insights into the complex signaling networks governing cellular responses to hypoxia.

HIF-1α Gel Shift Oligonucleotides References:

1. Hypoxia induces an autocrine-paracrine survival pathway via platelet-derived growth factor (PDGF)-B/PDGF-beta receptor/phosphatidylinositol 3-kinase/Akt signaling in RN46A neuronal cells.  |  Zhang, SX., et al. 2003. FASEB J. 17: 1709-11. PMID: 12958184
2. Effects of decreased insulin-like growth factor-1 stimulation on hypoxia inducible factor 1-alpha protein synthesis and function during cutaneous repair in diabetic mice.  |  Yu, DH., et al. 2007. Wound Repair Regen. 15: 628-35. PMID: 17971008
3. Anti-inflammatory effects of pigment epithelium-derived factor in diabetic nephropathy.  |  Wang, JJ., et al. 2008. Am J Physiol Renal Physiol. 294: F1166-73. PMID: 18322021
4. Placenta growth factor augments endothelin-1 and endothelin-B receptor expression via hypoxia-inducible factor-1 alpha.  |  Patel, N., et al. 2008. Blood. 112: 856-65. PMID: 18411415
5. Ferulic acid augments angiogenesis via VEGF, PDGF and HIF-1 alpha.  |  Lin, CM., et al. 2010. J Nutr Biochem. 21: 627-33. PMID: 19443196
6. Myeloid cell leukemia-1 (Mc1-1) is a candidate target gene of hypoxia-inducible factor-1 (HIF-1) in the testis.  |  Palladino, MA., et al. 2012. Reprod Biol Endocrinol. 10: 104. PMID: 23216940
7. Advanced glycated end-products affect HIF-transcriptional activity in renal cells.  |  Bondeva, T., et al. 2013. Mol Endocrinol. 27: 1918-33. PMID: 24030251
8. Simvastatin and downstream inhibitors circumvent constitutive and stromal cell-induced resistance to doxorubicin in IGHV unmutated CLL cells.  |  Rigoni, M., et al. 2015. Oncotarget. 6: 29833-46. PMID: 26284584
9. Alcoholic hepatitis accelerates early hepatobiliary cancer by increasing stemness and miR-122-mediated HIF-1α activation.  |  Ambade, A., et al. 2016. Sci Rep. 6: 21340. PMID: 26888602
10. Hepatocellular carcinoma is accelerated by NASH involving M2 macrophage polarization mediated by hif-1αinduced IL-10.  |  Ambade, A., et al. 2016. Oncoimmunology. 5: e1221557. PMID: 27853646
11. MicroRNA 122, Regulated by GRLH2, Protects Livers of Mice and Patients From Ethanol-Induced Liver Disease.  |  Satishchandran, A., et al. 2018. Gastroenterology. 154: 238-252.e7. PMID: 28987423
12. Macrophage-Specific Hypoxia-Inducible Factor-1α Contributes to Impaired Autophagic Flux in Nonalcoholic Steatohepatitis.  |  Wang, X., et al. 2019. Hepatology. 69: 545-563. PMID: 30102772
13. Baicalein resensitizes tamoxifen-resistant breast cancer cells by reducing aerobic glycolysis and reversing mitochondrial dysfunction via inhibition of hypoxia-inducible factor-1α.  |  Chen, Y., et al. 2021. Clin Transl Med. 11: e577. PMID: 34841716