TFEB Gel Shift Oligonucleotides: sc-2621

TFEB Gel Shift Oligonucleotides are short DNA sequences meticulously crafted for use in gel shift assays, a foundational technique in molecular biology research aimed at investigating protein-DNA interactions. The acronym "TFEB" refers to the transcription factor EB, a master regulator of lysosomal biogenesis, autophagy, and cellular metabolism. TFEB belongs to the microphthalmia/transcription factor E (MiT/TFE) family of transcription factors and plays a crucial role in coordinating the expression of genes involved in lysosomal function and autophagy in response to cellular stress and nutrient availability. Upon activation, TFEB translocates to the nucleus and binds to specific DNA sequences, known as coordinated lysosomal expression and regulation (CLEAR) elements, within the promoters of target genes, thereby modulating their transcriptional activity and influencing cellular homeostasis. By employing TFEB Gel Shift Oligonucleotides in gel shift assays, researchers can explore the binding kinetics, specificity, and affinity of TFEB to its target DNA sequences under various experimental conditions. This technique facilitates the elucidation of the molecular mechanisms underlying TFEB-mediated gene regulation and provides insights into the complex signaling networks governing lysosomal function, autophagy, and cellular metabolism.

TFEB Gel Shift Oligonucleotides References:

1. Transcription factor network downstream of protease activated receptors (PARs) modulating mouse bladder inflammation.  |  Saban, R., et al. 2007. BMC Immunol. 8: 17. PMID: 17705868
2. Coxsackievirus B3 targets TFEB to disrupt lysosomal function.  |  Mohamud, Y., et al. 2021. Autophagy. 17: 3924-3938. PMID: 33691586