Brn-3 Gel Shift Oligonucleotides: sc-2595

Brn-3 Gel Shift Oligonucleotides are short DNA sequences meticulously designed for use in gel shift assays, a foundational technique in molecular biology research aimed at probing protein-DNA interactions. The name "Brn-3" refers to the Brn-3 family of transcription factors, including Brn-3a (POU4F1), Brn-3b (POU4F2), and Brn-3c (POU4F3), which play crucial roles in the regulation of gene expression, particularly in the development and function of sensory neurons. These transcription factors belong to the POU domain family, characterized by a conserved DNA-binding domain known as the POU domain. Brn-3 factors are involved in diverse cellular processes, including neuronal differentiation, survival, and axonal pathfinding. Upon activation, Brn-3 factors translocate to the nucleus and bind to specific DNA sequences, known as Brn-3 response elements (BREs), within the promoters of target genes, thereby modulating their transcriptional activity. By employing Brn-3 Gel Shift Oligonucleotides in gel shift assays, researchers investigate the binding kinetics, specificity, and affinity of Brn-3 factors to their target DNA sequences under various experimental conditions. This technique facilitates the elucidation of the molecular mechanisms underlying Brn-3-mediated gene regulation and provides insights into the complex signaling networks governing neuronal development and function.