c-Myb Gel Shift Oligonucleotides: sc-2583

c-Myb Gel Shift Oligonucleotides are short DNA sequences meticulously designed for use in gel shift assays, a foundational technique in molecular biology research aimed at exploring protein-DNA interactions. The name "c-Myb" refers to the cellular homolog of the avian myeloblastosis viral oncogene v-Myb, encoding the c-Myb transcription factor, a crucial regulator of cell proliferation, differentiation, and apoptosis. c-Myb is a member of the Myb family of transcription factors, characterized by a conserved DNA-binding domain known as the Myb DNA-binding domain. Upon activation, c-Myb translocates to the nucleus and binds to specific DNA sequences, known as Myb-binding sites, within the promoters of target genes, thereby modulating their transcriptional activity. By utilizing c-Myb Gel Shift Oligonucleotides in gel shift assays, researchers can investigate the binding kinetics, specificity, and affinity of c-Myb to its target DNA sequences under various experimental conditions. This technique facilitates the elucidation of the molecular mechanisms underlying c-Myb-mediated gene regulation and provides insights into the complex signaling networks governing cellular processes such as hematopoiesis, immune response, and oncogenesis.

c-Myb Gel Shift Oligonucleotides References:

1. Identification and characterization of the hematopoietic cell-specific enhancer-like element of the mouse hex gene.  |  Sato, A., et al. 2004. J Biochem. 135: 259-68. PMID: 15047729
2. The functional -443T/C osteopontin promoter polymorphism influences osteopontin gene expression in melanoma cells via binding of c-Myb transcription factor.  |  Schultz, J., et al. 2009. Mol Carcinog. 48: 14-23. PMID: 18459127