NFATc Gel Shift Oligonucleotides: sc-2577

NFATc Gel Shift Oligonucleotides are short DNA sequences meticulously crafted for application in gel shift assays, a foundational technique in molecular biology research aimed at elucidating protein-DNA interactions. The nomenclature "NFATc" refers to Nuclear Factor of Activated T cells (NFAT), specifically the cytoplasmic isoform (NFATc), a vital transcription factor in immune cell activation and numerous cellular processes, including immune response modulation and development. Upon stimulation of T cell receptors or other immune signaling pathways, NFATc translocates to the nucleus and binds to specific DNA sequences, known as NFAT binding sites, within the promoters of target genes, thereby regulating their transcriptional activity. By utilizing NFATc Gel Shift Oligonucleotides in gel shift assays, researchers investigate the binding kinetics, specificity, and affinity of NFATc to its target DNA sequences under diverse experimental conditions. This technique facilitates the elucidation of the molecular mechanisms underlying NFATc-mediated gene regulation and provides insights into the signaling networks governing immune cell activation and immune responses.

NFATc Gel Shift Oligonucleotides References:

1. The calcineurin-NFAT pathway and muscle fiber-type gene expression.  |  Swoap, SJ., et al. 2000. Am J Physiol Cell Physiol. 279: C915-24. PMID: 11003571
2. Uridine 5′-diphosphate induces chemokine expression in microglia and astrocytes through activation of the P2Y6 receptor.  |  Kim, B., et al. 2011. J Immunol. 186: 3701-9. PMID: 21317391
3. Nuclear factor of activated T-cells (NFAT)C2 inhibits Notch receptor signaling in osteoblasts.  |  Zanotti, S., et al. 2013. J Biol Chem. 288: 624-32. PMID: 23166323