Stat3 Gel Shift Oligonucleotides: sc-2571

Stat3 Gel Shift Oligonucleotides are short DNA sequences meticulously engineered for utilization in gel shift assays, a fundamental technique in molecular biology research aimed at probing protein-DNA interactions. These oligonucleotides derive their name from Signal Transducer and Activator of Transcription 3 (Stat3), a transcription factor critical in cytokine signaling pathways and cellular processes such as proliferation, differentiation, and immune response. Upon activation by cytokines like interleukins and growth factors, Stat3 translocates to the nucleus and binds to specific DNA sequences, known as Stat3 binding sites, within the promoters of target genes, modulating their transcriptional activity. By employing Stat3 Gel Shift Oligonucleotides in gel shift assays, researchers delve into the binding kinetics, specificity, and affinity of Stat3 to its target DNA sequences under diverse experimental conditions. This technique enables the elucidation of the molecular mechanisms underlying Stat3-mediated gene regulation and provides insights into the complex signaling networks governing cellular responses to cytokine signaling.

Stat3 Gel Shift Oligonucleotides References:

1. Role of STAT3 and C/EBP in cytokine-dependent expression of the mouse serum amyloid P-component (SAP) and C-reactive protein (CRP) genes.  |  Ochrietor, JD., et al. 2000. Cytokine. 12: 888-99. PMID: 10880233
2. Reproductive hormone-induced, STAT3-mediated interleukin 6 action in normal and malignant human ovarian surface epithelial cells.  |  Syed, V., et al. 2002. J Natl Cancer Inst. 94: 617-29. PMID: 11959895
3. Interleukin 6/gp130-dependent pathways are protective during chronic liver diseases.  |  Streetz, KL., et al. 2003. Hepatology. 38: 218-29. PMID: 12830005
4. Lack of gp130 expression in hepatocytes promotes liver injury.  |  Streetz, KL., et al. 2003. Gastroenterology. 125: 532-43. PMID: 12891556
5. The human hepatocyte growth factor (HGF) gene is transcriptionally activated by leukemia inhibitory factor through the Stat binding element.  |  Tomida, M. and Saito, T. 2004. Oncogene. 23: 679-86. PMID: 14647442
6. STAT3 is constitutively activated and supports cell survival in association with survivin expression in gastric cancer cells.  |  Kanda, N., et al. 2004. Oncogene. 23: 4921-9. PMID: 15077160
7. Epigenetic modification of SOCS-1 differentially regulates STAT3 activation in response to interleukin-6 receptor and epidermal growth factor receptor signaling through JAK and/or MEK in head and neck squamous cell carcinomas.  |  Lee, TL., et al. 2006. Mol Cancer Ther. 5: 8-19. PMID: 16432158
8. A novel RBP-J kappa-dependent switch from C/EBP beta to C/EBP zeta at the C/EBP binding site on the C-reactive protein promoter.  |  Singh, PP., et al. 2007. J Immunol. 178: 7302-9. PMID: 17513780
9. Epstein-Barr virus-derived EBNA2 regulates STAT3 activation.  |  Muromoto, R., et al. 2009. Biochem Biophys Res Commun. 378: 439-43. PMID: 19032945
10. Calyculin A reveals serine/threonine phosphatase protein phosphatase 1 as a regulatory nodal point in canonical signal transducer and activator of transcription 3 signaling of human microvascular endothelial cells.  |  Zgheib, C., et al. 2012. J Interferon Cytokine Res. 32: 87-94. PMID: 22142222
11. Alantolactone selectively suppresses STAT3 activation and exhibits potent anticancer activity in MDA-MB-231 cells.  |  Chun, J., et al. 2015. Cancer Lett. 357: 393-403. PMID: 25434800
12. Schistosoma mansoni Egg-Secreted Antigens Activate Hepatocellular Carcinoma-Associated Transcription Factors c-Jun and STAT3 in Hamster and Human Hepatocytes.  |  Roderfeld, M., et al. 2020. Hepatology. 72: 626-641. PMID: 30053321
13. TRIM8-driven transcriptomic profile of neural stem cells identified glioma-related nodal genes and pathways.  |  Venuto, S., et al. 2019. Biochim Biophys Acta Gen Subj. 1863: 491-501. PMID: 30528352