Stat5/Stat6 Gel Shift Oligonucleotides: sc-2567

Stat5/Stat6 Gel Shift Oligonucleotides are short DNA sequences meticulously designed for applications in gel shift assays, a pivotal technique in molecular biology research used to explore protein-DNA interactions. These oligonucleotides are tailored based on the consensus binding sequences of Signal Transducer and Activator of Transcription 5 (Stat5) and Signal Transducer and Activator of Transcription 6 (Stat6), transcription factors crucial in cytokine signaling pathways and cellular processes such as proliferation, differentiation, and immune response. Upon activation by cytokines such as interleukins and growth factors, Stat5 and Stat6 translocate to the nucleus and bind to specific DNA sequences, known as Stat5 and Stat6 binding sites, within the promoters of target genes, thereby modulating their transcriptional activity. By utilizing Stat5/Stat6 Gel Shift Oligonucleotides in gel shift assays, researchers can investigate the binding kinetics, specificity, and affinity of Stat5 and Stat6 to their target DNA sequences under diverse experimental conditions. This technique facilitates the elucidation of the molecular mechanisms underlying Stat5- and Stat6-mediated gene regulation and provides insights into the complex signaling networks governing cellular responses to cytokine signaling.