Stat5 Gel Shift Oligonucleotides: sc-2565

Stat5 Gel Shift Oligonucleotides are short DNA sequences meticulously crafted for applications in gel shift assays, a crucial technique in molecular biology research used to explore protein-DNA interactions. These oligonucleotides are designed based on the consensus binding sequence of Signal Transducer and Activator of Transcription 5 (Stat5), a transcription factor pivotal in cytokine signaling pathways and cellular processes such as proliferation, differentiation, and immune response. Upon activation by cytokines such as interleukins and growth factors, Stat5 translocates to the nucleus and binds to specific DNA sequences, known as Stat5 binding sites, within the promoters of target genes, thereby modulating their transcriptional activity. By employing Stat5 Gel Shift Oligonucleotides in gel shift assays, researchers can investigate the binding kinetics, specificity, and affinity of Stat5 to its target DNA sequences under diverse experimental conditions. This technique facilitates the elucidation of the molecular mechanisms underlying Stat5-mediated gene regulation and provides insights into the complex signaling networks governing cellular responses to cytokine signaling.

Stat5 Gel Shift Oligonucleotides References:

1. Conversion of threonine 757 to valine enhances Stat5a transactivation potential.  |  Gowri, PM., et al. 2001. J Biol Chem. 276: 10485-91. PMID: 11133982
2. Characterization of a rat uterine cell line, U(III) cells: prolactin (PRL) expression and endogenous regulation of PRL-dependent genes; estrogen receptor beta, alpha(2)-macroglobulin, and decidual PRL involving the Jak2 and Stat5 pathway.  |  Prigent-Tessier, A., et al. 2001. Endocrinology. 142: 1242-50. PMID: 11181541
3. PRL, placental lactogen, and GH induce NA(+)/taurocholate-cotransporting polypeptide gene expression by activating signal transducer and activator of transcription-5 in liver cells.  |  Cao, J., et al. 2001. Endocrinology. 142: 4212-22. PMID: 11564677
4. Pharmacologic mitogen-activated protein/extracellular signal-regulated kinase kinase/mitogen-activated protein kinase inhibitors interact synergistically with STI571 to induce apoptosis in Bcr/Abl-expressing human leukemia cells.  |  Yu, C., et al. 2002. Cancer Res. 62: 188-99. PMID: 11782377
5. Interleukin-6 inhibits hepatic growth hormone signaling via upregulation of Cis and Socs-3.  |  Denson, LA., et al. 2003. Am J Physiol Gastrointest Liver Physiol. 284: G646-54. PMID: 12519742
6. Thiol alkylation inhibits the mitogenic effects of platelet-derived growth factor and renders it proapoptotic via activation of STATs and p53 and induction of expression of caspase1 and p21(waf1/cip1).  |  Bhanoori, M., et al. 2003. Oncogene. 22: 117-30. PMID: 12527914
7. Distinct regions of the interleukin-7 receptor regulate different Bcl2 family members.  |  Jiang, Q., et al. 2004. Mol Cell Biol. 24: 6501-13. PMID: 15226449
8. Novel role for STAT-5B in the regulation of Hsp27-FGF-2 axis facilitating thrombin-induced vascular smooth muscle cell growth and motility.  |  Cao, H., et al. 2006. Circ Res. 98: 913-22. PMID: 16527988
9. Transcriptional activation of mouse TCR Jgamma4 germline promoter by STAT5.  |  Masui, N., et al. 2008. Mol Immunol. 45: 849-55. PMID: 17664008
10. The signal transducer STAT5 inhibits plasmacytoid dendritic cell development by suppressing transcription factor IRF8.  |  Esashi, E., et al. 2008. Immunity. 28: 509-20. PMID: 18342552
11. A 5′ distal palindrome within the mouse mammary tumor virus-long terminal repeat recruits a mammary gland-specific complex and is required for a synergistic response to progesterone plus prolactin.  |  Morabito, JE., et al. 2008. J Mol Endocrinol. 41: 75-90. PMID: 18524869
12. Negative regulation of HIV-1 transcription by a heterodimeric NF-κB1/p50 and C-terminally truncated STAT5 complex.  |  Della Chiara, G., et al. 2011. J Mol Biol. 410: 933-43. PMID: 21763497
13. Regulation of the rat liver sodium-dependent bile acid cotransporter gene by prolactin. Mediation of transcriptional activation by Stat5.  |  Ganguly, TC., et al. 1997. J Clin Invest. 99: 2906-14. PMID: 9185514
14. Clonal variability in beta-globin mRNA content in an interleukin-3-dependent bone marrow cell line transfected with the erythropoietin receptor before and after stimulation with erythropoietin.  |  Ishiguro, K. and Sartorelli, AC. 1997. Blood. 90: 2273-81. PMID: 9310478
15. Male-specific transcription initiation of the C4-Slp gene in mouse liver follows activation of STAT5.  |  Varin-Blank, N., et al. 1998. Proc Natl Acad Sci U S A. 95: 8750-5. PMID: 9671750