Ect2 (H-300) Antibody: sc-25637

Ect2 Background Information
Numerous cellular functions such as proliferation, differentiation, apoptosis, vesicular trafficking, nuclear transport and cytoskeletal organization are controlled by GTPases (1,2). It has become increasingly clear that GTPases act in cascades in which their activities are linked by GTPase-activating proteins (GAPs) and guanine nucleotide exchange factors (GEFs) (1,2). In a search for new epithelial cell-specific oncogenes using a highly efficient cDNA expression cloning system (3), the ost oncogene was isolated from rat osteosarcoma cells (4). The Ost proto-oncogene protein contains DH (5,6) and PH (7) domains and catalyzes guanine nucleotide exchange on RhoA and Cdc42 and interacts specifically with the GTP-bound form of Rac1. A similar protein, Ect2, specifically interacts with Rho and Rac proteins in vitro. Ect2 shares sequence homology with the 255 amino acid central core of the breakpoint cluster gene, bcr, as well as with yeast CDC24 and the Dbl oncogene, all of which have been shown to modulate the function of small Rho-like GTP binding proteins (8). The Ect2 contains both PH and DH domains (8).