NF-1 Gel Shift Oligonucleotides: sc-2553

NF-1 Gel Shift Oligonucleotides are short DNA sequences meticulously designed for use in gel shift assays, a foundational technique in molecular biology research employed to investigate protein-DNA interactions. The name "NF-1" corresponds to the nuclear factor 1 (NF-1), a transcription factor recognized for its pivotal role in regulating gene expression and cellular processes such as development, differentiation, and proliferation. NF-1 binds to specific DNA sequences, known as NF-1 binding sites, within the promoters and enhancers of target genes, thereby modulating their transcriptional activity. By utilizing NF-1 Gel Shift Oligonucleotides in gel shift assays, researchers explore the binding kinetics, specificity, and affinity of NF-1 to NF-1 binding sites under various experimental conditions. This technique enables the elucidation of the molecular mechanisms underlying NF-1-mediated gene regulation and provides insights into the signaling networks governing cellular processes.

NF-1 Gel Shift Oligonucleotides References:

1. Transcriptional regulation of cell type-specific expression of the TATA-less A subunit gene for human coagulation factor XIII.  |  Kida, M., et al. 1999. J Biol Chem. 274: 6138-47. PMID: 10037697
2. Biochemical characterization of a nuclear factor that binds to NF1-like elements in the rat p53 promoter.  |  Lee, M., et al. 2000. J Cell Biochem. 78: 1-7. PMID: 10797561
3. A functional NF-kappaB binding site in the human papillomavirus type 16 long control region.  |  Fontaine, V., et al. 2000. Virology. 272: 40-9. PMID: 10873747
4. Transcriptional regulation of the human cystathionine beta-synthase -1b basal promoter: synergistic transactivation by transcription factors NF-Y and Sp1/Sp3.  |  Ge, Y., et al. 2001. Biochem J. 357: 97-105. PMID: 11415440
5. Transcriptional regulation of human placental leucine aminopeptidase/oxytocinase gene.  |  Ito, T., et al. 2001. Mol Hum Reprod. 7: 887-94. PMID: 11517297
6. Characterization of human cathepsin L promoter and identification of binding sites for NF-Y, Sp1 and Sp3 that are essential for its activity.  |  Jean, D., et al. 2002. Biochem J. 361: 173-84. PMID: 11742542
7. Organic dust activates NF-kappaB in lung epithelial cells.  |  Lidεn, J., et al. 2003. Respir Med. 97: 882-92. PMID: 12924514
8. Increased cytochrome P450 17alpha-hydroxylase promoter function in theca cells isolated from patients with polycystic ovary syndrome involves nuclear factor-1.  |  Wickenheisser, JK., et al. 2004. Mol Endocrinol. 18: 588-605. PMID: 14684846
9. Genetic and biochemical analysis of cis regulatory elements within the keratinocyte enhancer region of the human papillomavirus type 31 upstream regulatory region during different stages of the viral life cycle.  |  Sen, E., et al. 2004. J Virol. 78: 612-29. PMID: 14694093
10. The human involucrin gene is transcriptionally repressed through a tissue-specific silencer element recognized by Oct-2.  |  Azuara-Liceaga, E., et al. 2004. Biochem Biophys Res Commun. 318: 361-71. PMID: 15120610
11. Pentoxifylline, pentifylline, and interferons decrease type I and III procollagen mRNA levels in dermal fibroblasts: evidence for mediation by nuclear factor 1 down-regulation.  |  Duncan, MR., et al. 1995. J Invest Dermatol. 104: 282-6. PMID: 7530274
12. Transforming growth factor-beta 1 regulation of bone sialoprotein gene transcription: identification of a TGF-beta activation element in the rat BSP gene promoter.  |  Ogata, Y., et al. 1997. J Cell Biochem. 65: 501-12. PMID: 9178100
13. Mechanisms in the transcriptional regulation of bradykinin B1 receptor gene expression. Identification of a minimum cell-type specific enhancer.  |  Yang, X., et al. 1998. J Biol Chem. 273: 10763-70. PMID: 9553142
14. Multiple domains for initiator binding proteins TFII-I and YY-1 are present in the initiator and upstream regions of the rat XDH/XO TATA-less promoter.  |  Clark, MP., et al. 1998. Nucleic Acids Res. 26: 2813-20. PMID: 9592172
15. Transcriptional activation of the alpha1(I) procollagen gene and up-regulation of alpha1(I) and alpha1(III) procollagen messenger RNA in dermal fibroblasts from tight skin 2 mice.  |  Christner, PJ., et al. 1998. Arthritis Rheum. 41: 2132-42. PMID: 9870870