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- rabbit polyclonal IgG, 200 µg/ml; also available as goat IgG, 200 µg/ml, sc-255-G
- epitope mapping at the C-terminus of GRB2 of human origin
- recommended for detection of GRB2 p25 of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
- blocking peptide, sc-255 P
- agarose conjugate for IP studies, sc-255 AC, 500 µg/0.25 ml agarose
- phycoerythrin conjugate for intracellular FCM, sc-255 PE, 100 tests
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GRB2 Background Information
The superfamily of GTP binding proteins, of which Ras proteins are prototypes, has been implicated in a broad range of biological activities. A family of guanine nucleotide releasing factors (GRFs) activate Ras in mammalian cells and growth factor receptor-bound protein 2 (GRB2), an adaptor protein (also referred to as Sem 5) that appears to mediate the interaction of GRFs with activated receptor molecules. GRB2 forms a complex with activated EGFR (epidermal growth factor receptor) and the Ras-specific guanine nucleotide exchange factor SOS1, and, together, they regulate the growth factor-induced activation of Ras. GRB2 exhibits both structural and functional homology to the C. elegans protein sem-5. GRB2 is necessary during embryogenesis for the differentiation of endodermal cells and formation of the epiblast.
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See how others have used GRB2 (C-23): sc-255 antibody and or GRB2 (C-23) antibody conjugates.
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GRB2 (C-23)
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GRB2 (C-23): sc-255. Western blot analysis of GRB2 expression in PC-12 (A) and KNRK (B) whole cell lysates.
GRB2 (C-23): sc-255. Immunofluorescence staining of methanol-fixed HeLa cells showing specific staining of membrane ruffles (A). Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing membrane staining of mucosal epithelia (B).
GRB2 (C-23)-G: sc-255-G. Western blot analysis of mouse recombinant GRB2 fusion protein. Western blot analysis of GRB2 expression in Jurkat immunoprecipitated with GRB2 (1-68): sc-503 and detected with GRB2 (G-23): sc-255. GRB2 (C-23) PE: sc-255 PE. Intracellular FCM analysis of fixed and permeabilized PC-12 cells. Black line histogram represents the isotype control, normal rabbit IgG: sc-3871. GRB2 (C-23)-G: sc-255-G. Western blot analysis of GRB2 expression in Jurkat whole cell lysate. GRB2 (C-23): sc-255. Western blot analysis of GRB2 expression in Jurkat whole cell lysate.
GRB2 (C-23): sc-255. Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.
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