GR Gel Shift Oligonucleotides: sc-2545

GR Gel Shift Oligonucleotides are short DNA sequences specifically designed for use in gel shift assays, a cornerstone technique in molecular biology research aimed at studying protein-DNA interactions. The name "GR" stands for Glucocorticoid Receptor, indicating the consensus binding sequence recognized by the glucocorticoid receptor (GR) transcription factor. GR is a member of the nuclear receptor superfamily and plays a central role in mediating the effects of glucocorticoid hormones on gene expression. Upon activation by glucocorticoids, GR translocates to the nucleus and binds to specific DNA sequences, known as glucocorticoid response elements (GREs), within the promoters of target genes, thereby modulating their transcriptional activity. By utilizing GR Gel Shift Oligonucleotides in gel shift assays, researchers can investigate the binding kinetics, specificity, and affinity of GR to GREs under various experimental conditions. This technique facilitates the elucidation of the molecular mechanisms underlying GR-mediated gene regulation and provides insights into the complex signaling networks that govern cellular responses to glucocorticoid signaling.

GR Gel Shift Oligonucleotides References:

1. Multiple domains of melatonin receptor are involved in the regulation of glucocorticoid receptor-induced gene expression.  |  Persengiev, SP. 1999. J Steroid Biochem Mol Biol. 68: 181-7. PMID: 10416832
2. Glucocorticoid pretreatment induces cytokine overexpression and nuclear factor-kappaB activation in macrophages.  |  Smyth, GP., et al. 2004. J Surg Res. 116: 253-61. PMID: 15013364