GAS/ISRE Gel Shift Oligonucleotides: sc-2537

GAS/ISRE Gel Shift Oligonucleotides are short DNA sequences tailored for utilization in gel shift assays, a foundational tool in molecular biology research employed to examine protein-DNA interactions. The name "GAS/ISRE" refers to the consensus binding sequences recognized by the transcription factors STAT (Signal Transducer and Activator of Transcription) and ISGF3 (Interferon-Stimulated Gene Factor 3), respectively. These transcription factors play critical roles in mediating cellular responses to cytokines and interferons, respectively, thereby regulating gene expression involved in immune response, cell proliferation, and antiviral defense. GAS/ISRE Gel Shift Oligonucleotides are instrumental in gel shift assays, allowing researchers to investigate the binding kinetics, specificity, and affinity of STAT and ISGF3 to their respective target DNA sequences under various experimental conditions. This technique facilitates the elucidation of the molecular mechanisms underlying STAT and ISGF3-mediated gene regulation and provides insights into the signaling networks governing cellular responses to cytokine and interferon signaling.

GAS/ISRE Gel Shift Oligonucleotides References:

1. Characterization of insulin-like growth factor-1-induced activation of the JAK/STAT pathway in rat cardiomyocytes.  |  Takahashi, T., et al. 1999. Circ Res. 85: 884-91. PMID: 10559134
2. Altered T cell development in human thymoma is related to impairment of MHC class II transactivator expression induced by interferon-gamma (IFN-gamma).  |  Kadota, Y., et al. 2000. Clin Exp Immunol. 121: 59-68. PMID: 10886240
3. Trichodion, a new inhibitor of inflammatory signal transduction pathways from a Trichosporiella species.  |  Erkel, G. 2000. FEBS Lett. 477: 219-23. PMID: 10908724
4. Sporogen, S14-95, and S-curvularin, three inhibitors of human inducible nitric-oxide synthase expression isolated from fungi.  |  Yao, Y., et al. 2003. Mol Pharmacol. 63: 383-91. PMID: 12527810
5. S14-95, a novel inhibitor of the JAK/STAT pathway from a Penicillium species.  |  Erkel, G., et al. 2003. J Antibiot (Tokyo). 56: 337-43. PMID: 12817806
6. Nuclear factor-kappaB motif and interferon-alpha-stimulated response element co-operate in the activation of guanylate-binding protein-1 expression by inflammatory cytokines in endothelial cells.  |  Naschberger, E., et al. 2004. Biochem J. 379: 409-20. PMID: 14741045
7. Phomol, a new antiinflammatory metabolite from an endophyte of the medicinal plant Erythrina crista-galli.  |  Weber, D., et al. 2004. J Antibiot (Tokyo). 57: 559-63. PMID: 15580955
8. Proteasome-mediated degradation of STAT1alpha following infection of macrophages with Leishmania donovani.  |  Forget, G., et al. 2005. J Biol Chem. 280: 30542-9. PMID: 15983048
9. Metabolites from endophytes of the medicinal plant Erythrina crista-galli.  |  Weber, D., et al. 2005. Z Naturforsch C J Biosci. 60: 467-77. PMID: 16042349
10. Inhibitors of inducible NO synthase expression: total synthesis of (S)-curvularin and its ring homologues.  |  Elzner, S., et al. 2008. ChemMedChem. 3: 924-39. PMID: 18366037
11. Novel pathway of insulin signaling involving Stat1alpha in Hep3B cells.  |  Chuang, LM., et al. 1997. Biochem Biophys Res Commun. 235: 317-20. PMID: 9199189