SIE Gel Shift Oligonucleotides: sc-2535
- consensus binding site (Sis-inducible element) for Sis-inducible factor; supplied as 500 ng double-stranded DNA; sc-2535
- also available as mutant oligonucleotide with a "TTC"→"CCA" substitution in the DNA binding region; sc-2536
- 5′-GTG CAT TTC CCG TAA ATC TTG TCT ACA-3′
- also available as an agarose conjugate for use in purifying or enriching specific transcription factors; 15 µg in 0.25 ml packed beads; sufficient for 10 assays; use 25 µl beads per 0.5 - 1 mg total cell lysate protein, sc-2505 AC
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SEE ALSO...
SIE Gel Shift Oligonucleotides are short sequences of DNA designed for use in gel shift assays, a foundational technique in molecular biology research aimed at studying protein-DNA interactions. The name "SIE" stands for Sis-Inducible Element, referring to the consensus binding sequence recognized by the transcription factor Sis (also known as Stat3) in the promoter regions of target genes. Stat3 is a key player in signal transduction pathways activated by various cytokines and growth factors, and its activation leads to the modulation of gene expression involved in processes such as cell proliferation, differentiation, and immune response. SIE Gel Shift Oligonucleotides are utilized in gel shift assays to investigate the binding kinetics, specificity, and affinity of Stat3 to SIE motifs under different experimental conditions. This technique enables researchers to explain the molecular mechanisms underlying Stat3-mediated gene regulation and provides insights into the complex signaling networks that govern cellular responses to extracellular stimuli.
SIE Gel Shift Oligonucleotides References:
- Angiotensin blockade inhibits SIF DNA binding activities via STAT3 after myocardial infarction. | Omura, T., et al. 2000. J Mol Cell Cardiol. 32: 23-33. PMID: 10652187
- Interleukin-6-induced tethering of STAT3 to the LAP/C/EBPbeta promoter suggests a new mechanism of transcriptional regulation by STAT3. | Niehof, M., et al. 2001. J Biol Chem. 276: 9016-27. PMID: 11114305
- Myocardial ischemia activates the JAK-STAT pathway through angiotensin II signaling in in vivo myocardium of rats. | Omura, T., et al. 2001. J Mol Cell Cardiol. 33: 307-16. PMID: 11162135
- Steel factor induces serine phosphorylation of Stat3 in human growth factor-dependent myeloid cell lines. | Gotoh, A., et al. 1996. Blood. 88: 138-45. PMID: 8704168
- Expression of v-src in mammary epithelial cells induces transcription via STAT3. | Smith, PD. and Crompton, MR. 1998. Biochem J. 331 (Pt 2): 381-5. PMID: 9531474
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SIE consensus oligonucleotide | sc-2535 | 500 ng/25 µl | $49.00 | |||
SIE consensus oligonucleotide AC | sc-2535 AC | 15 µg/0.25 ml | $153.00 | |||
SIE mutant oligonucleotide | sc-2536 | 500 ng/25 µl | $49.00 | |||
SIE mutant oligonucleotide AC | sc-2536 AC | 15 µg/0.25 ml | $153.00 |