YY1 Gel Shift Oligonucleotides: sc-2533

YY1 Gel Shift Oligonucleotides are short sequences of DNA meticulously crafted for use in gel shift assays, a cornerstone technique in molecular biology employed to scrutinize protein-DNA interactions. Named after the Yin Yang 1 (YY1) transcription factor, these oligonucleotides are derived from the consensus binding sequence recognized by YY1, a multifunctional transcription factor with diverse roles in gene regulation, cellular proliferation, differentiation, and apoptosis. YY1 exerts its regulatory influence by binding to specific DNA sequences, termed YY1 motifs, within the promoters and enhancers of target genes, thereby modulating their transcriptional activity. By utilizing YY1 Gel Shift Oligonucleotides in gel shift assays, researchers can delve into the binding kinetics, specificity, and affinity of YY1 to YY1 motifs under varied experimental conditions. This technique facilitates the elucidation of the molecular mechanisms underlying YY1-mediated gene regulation and provides insights into the complex signaling networks governing cellular processes such as development, differentiation, and oncogenesis.

YY1 Gel Shift Oligonucleotides References:

1. YY1 binds five cis-elements and trans-activates the myeloid cell-restricted gp91(phox) promoter.  |  Jacobsen, BM. and Skalnik, DG. 1999. J Biol Chem. 274: 29984-93. PMID: 10514482
2. Nuclear factor of activated T cells and YY1 combine to repress IL-5 expression in a human T-cell line.  |  Schwenger, GT., et al. 1999. J Allergy Clin Immunol. 104: 820-7. PMID: 10518827
3. The transcriptional regulator Yin Yang 1 activates the myelin PLP gene.  |  Berndt, JA., et al. 2001. J Neurochem. 77: 935-42. PMID: 11331422
4. YY1 represses vitamin D receptor-mediated 25-hydroxyvitamin D(3)24-hydroxylase transcription: relief of repression by CREB-binding protein.  |  Raval-Pandya, M., et al. 2001. Mol Endocrinol. 15: 1035-46. PMID: 11376120
5. YY1 is a positive regulator of transcription of the Col1a1 gene.  |  Riquet, FB., et al. 2001. J Biol Chem. 276: 38665-72. PMID: 11514536
6. YY1 transcription factor down-regulates expression of CCR5, a major coreceptor for HIV-1.  |  Moriuchi, M. and Moriuchi, H. 2003. J Biol Chem. 278: 13003-7. PMID: 12571248
7. Yin-yang 1 negatively regulates the differentiation-specific transcription of mouse loricrin gene in undifferentiated keratinocytes.  |  Xu, X., et al. 2004. J Invest Dermatol. 123: 1120-6. PMID: 15610523
8. Exendin-4 induction of Egr-1 expression in INS-1 beta-cells: interaction of SRF, not YY1, with SRE site of rat Egr-1 promoter.  |  Kim, MJ., et al. 2008. J Cell Biochem. 104: 2261-71. PMID: 18446785
9. A common promoter variant of TBX21 is associated with allele specific binding to Yin-Yang 1 and reduced gene expression.  |  Li, JR., et al. 2011. Scand J Immunol. 73: 449-58. PMID: 21272048
10. YY1 negatively regulates mouse myelin proteolipid protein (Plp1) gene expression in oligodendroglial cells.  |  Zolova, OE. and Wight, PA. 2011. ASN Neuro. 3: PMID: 21973168
11. The nuclear matrix protein NMP-1 is the transcription factor YY1.  |  Guo, B., et al. 1995. Proc Natl Acad Sci U S A. 92: 10526-30. PMID: 7479833
12. YY1 represses beta-casein gene expression by preventing the formation of a lactation-associated complex.  |  Raught, B., et al. 1994. Mol Cell Biol. 14: 1752-63. PMID: 8114709
13. Identification and characterization of the human XIST gene promoter: implications for models of X chromosome inactivation.  |  Hendrich, BD., et al. 1997. Nucleic Acids Res. 25: 2661-71. PMID: 9185579
14. Regulation of RNA polymerase II-dependent transcription by poly(ADP-ribosyl)ation of transcription factors.  |  Oei, SL., et al. 1998. J Biol Chem. 273: 31644-7. PMID: 9822623
15. Identification of two repressor elements in the mouse alpha 2(I) collagen promoter.  |  Miao, K., et al. 1999. Arch Biochem Biophys. 361: 7-16. PMID: 9882423