GATA Gel Shift Oligonucleotides: sc-2531

GATA Gel Shift Oligonucleotides are short DNA sequences specifically tailored for use in gel shift assays, a fundamental tool in molecular biology for scrutinizing protein-DNA interactions. These oligonucleotides derive their name from the consensus binding sequence of the GATA family of transcription factors, renowned for their pivotal roles in regulating gene expression during development and hematopoiesis. GATA factors, including GATA-1, GATA-2, and GATA-3, bind to specific DNA sequences, known as GATA motifs, within the promoters and enhancers of target genes, modulating their transcriptional activity. By employing GATA Gel Shift Oligonucleotides in gel shift assays, researchers can delve into the binding kinetics, specificity, and affinity of GATA factors to GATA motifs under diverse experimental conditions. This technique enables the elucidation of the molecular mechanisms underlying GATA-mediated gene regulation and provides insights into the signaling networks governing cellular processes such as cell differentiation, proliferation, and lineage commitment.

GATA Gel Shift Oligonucleotides References:

1. Characterization of promoter 1B in the human glucocorticoid receptor gene.  |  Nunez, BS. and Vedeckis, WV. 2002. Mol Cell Endocrinol. 189: 191-9. PMID: 12039077
2. Regulation of the human brain natriuretic peptide gene by GATA-4.  |  He, Q., et al. 2002. Am J Physiol Endocrinol Metab. 283: E50-7. PMID: 12067842
3. GATA-4 and GATA-6 transcription factors: expression, immunohistochemical localization, and possible function in the porcine ovary.  |  Gillio-Meina, C., et al. 2003. Biol Reprod. 68: 412-22. PMID: 12533404
4. High-level activation by a duodenum-specific enhancer requires functional GATA binding sites.  |  Dusing, MR., et al. 2003. Am J Physiol Gastrointest Liver Physiol. 284: G1053-65. PMID: 12571085
5. TNF-alpha induces a decrease in eNOS promoter activity.  |  Neumann, P., et al. 2004. Am J Physiol Lung Cell Mol Physiol. 286: L452-9. PMID: 14555463
6. Extracellular signal-regulated kinase induces the megakaryocyte GPIIb/CD41 gene through MafB/Kreisler.  |  Sevinsky, JR., et al. 2004. Mol Cell Biol. 24: 4534-45. PMID: 15121870
7. [Up-regulation of transcription factors GATA-1 and GATA-2 induced by Panax notoginosides in hematopoietic cells].  |  Gao, RL., et al. 2004. Zhonghua Xue Ye Xue Za Zhi. 25: 281-4. PMID: 15182536
8. Calcineurin-nuclear factor of activated T cells pathway-dependent cardiac remodeling in mice deficient in guanylyl cyclase A, a receptor for atrial and brain natriuretic peptides.  |  Tokudome, T., et al. 2005. Circulation. 111: 3095-104. PMID: 15939815
9. GATA-1 mediates auto-regulation of Gfi-1B transcription in K562 cells.  |  Huang, DY., et al. 2005. Nucleic Acids Res. 33: 5331-42. PMID: 16177182
10. Panax notoginseng saponins induced up-regulation, phosphorylation and binding activity of MEK, ERK, AKT, PI-3K protein kinases and GATA transcription factors in hematopoietic cells.  |  Sun, X., et al. 2013. Chin J Integr Med. 19: 112-8. PMID: 23371459
11. A nuclear factor of activated T cell-like transcription factor in mast cells is involved in IL-5 gene regulation after IgE plus antigen stimulation.  |  Prieschl, EE., et al. 1995. J Immunol. 154: 6112-9. PMID: 7751652
12. The LIM-only protein Lmo2 is a bridging molecule assembling an erythroid, DNA-binding complex which includes the TAL1, E47, GATA-1 and Ldb1/NLI proteins.  |  Wadman, IA., et al. 1997. EMBO J. 16: 3145-57. PMID: 9214632
13. Cloning of rat and mouse aquaporin-2 gene promoters and identification of a negative cis-regulatory element.  |  Rai, T., et al. 1997. Am J Physiol. 273: F264-73. PMID: 9277587
14. Multiple factors interacting at the GATA sites of the gonadotropin-releasing hormone neuron-specific enhancer regulate gene expression.  |  Lawson, MA., et al. 1998. Mol Endocrinol. 12: 364-77. PMID: 9514154
15. CCAAT displacement protein (CDP/cut) binds a negative regulatory element in the human tryptophan hydroxylase gene.  |  Teerawatanasuk, N., et al. 1999. J Neurochem. 72: 29-39. PMID: 9886051