NF-E2 Gel Shift Oligonucleotides: sc-2527

NF-E2 Gel Shift Oligonucleotides are short DNA sequences designed for use in gel shift assays, a fundamental technique in molecular biology research for investigating protein-DNA interactions. These oligonucleotides are derived from the consensus binding sequence of the nuclear factor erythroid 2 (NF-E2), a transcription factor critical for regulating gene expression in erythroid cells and megakaryocytes. NF-E2 plays a central role in the transcriptional activation of genes involved in erythropoiesis, hemoglobin synthesis, and platelet production. Upon activation, NF-E2 binds to specific DNA sequences, known as Maf recognition elements (MAREs), within the promoters of target genes, thereby modulating their transcriptional activity. By utilizing NF-E2 Gel Shift Oligonucleotides in gel shift assays, researchers can explore the binding kinetics, specificity, and affinity of NF-E2 to MARE sequences under various experimental conditions. This technique enables the elucidation of the molecular mechanisms underlying NF-E2-mediated gene regulation and provides insights into the complex signaling networks that govern erythropoiesis and thrombopoiesis.

NF-E2 Gel Shift Oligonucleotides References:

1. Nrf2 mediates the induction of ferritin H in response to xenobiotics and cancer chemopreventive dithiolethiones.  |  Pietsch, EC., et al. 2003. J Biol Chem. 278: 2361-9. PMID: 12435735