C/EBP Gel Shift Oligonucleotides: sc-2525

C/EBP Gel Shift Oligonucleotides are short sequences of DNA designed for use in gel shift assays, a fundamental technique in molecular biology used to study protein-DNA interactions. These oligonucleotides are crafted based on the consensus binding sequence of the CCAAT/enhancer-binding protein (C/EBP), a family of transcription factors known for their critical roles in regulating gene expression and cellular differentiation. C/EBP proteins bind to specific DNA sequences, often located within enhancer or promoter regions of target genes, and modulate their transcriptional activity. By utilizing C/EBP Gel Shift Oligonucleotides in gel shift assays, researchers can explore the binding kinetics, specificity, and affinity of C/EBP proteins to their target DNA sequences under various experimental conditions. This technique enables the elucidation of the molecular mechanisms underlying C/EBP-mediated gene regulation and provides insights into the complex signaling networks that govern cellular processes such as metabolism, immune response, and adipogenesis.

C/EBP Gel Shift Oligonucleotides References:

1. Hepatocyte growth factor activates CCAAT enhancer binding protein and cell replication via PI3-kinase pathway.  |  Cho, MK. and Kim, SG. 2003. Hepatology. 37: 686-95. PMID: 12601366
2. Rapid, sequential activation of mitogen-activated protein kinases and transcription factors precedes proinflammatory cytokine mRNA expression in spleens of mice exposed to the trichothecene vomitoxin.  |  Zhou, HR., et al. 2003. Toxicol Sci. 72: 130-42. PMID: 12604842
3. Inhibition of lipopolysaccharide-inducible nitric oxide synthase, TNF-alpha and COX-2 expression by sauchinone effects on I-kappaBalpha phosphorylation, C/EBP and AP-1 activation.  |  Lee, AK., et al. 2003. Br J Pharmacol. 139: 11-20. PMID: 12746218
4. Induction of cyclooxygenase-2 by bovine type I collagen in macrophages via C/EBP and CREB activation by multiple cell signaling pathways.  |  Cho, MK., et al. 2004. Biochem Pharmacol. 67: 2239-50. PMID: 15163555
5. Interleukin-6-induced plasminogen gene expression in murine hepatocytes is mediated by transcription factor CCAAT/enhancer binding protein beta (C/EBPbeta).  |  Bannach, FG., et al. 2004. J Thromb Haemost. 2: 2205-12. PMID: 15613028
6. Glucocorticoids increase C/EBPbeta activity in the lung epithelium via phosphorylation.  |  Berg, T., et al. 2005. Biochem Biophys Res Commun. 334: 638-45. PMID: 16009338
7. 5′UTR of the neurogenic bHLH Nex1/MATH-2/NeuroD6 gene is regulated by two distinct promoters through CRE and C/EBP binding sites.  |  Uittenbogaard, M., et al. 2007. J Neurosci Res. 85: 1-18. PMID: 17075921
8. A novel RBP-J kappa-dependent switch from C/EBP beta to C/EBP zeta at the C/EBP binding site on the C-reactive protein promoter.  |  Singh, PP., et al. 2007. J Immunol. 178: 7302-9. PMID: 17513780
9. Stimulation of MAPK-phosphatase 1 gene expression by glucocorticoids occurs through a tethering mechanism involving C/EBP.  |  Johansson-Haque, K., et al. 2008. J Mol Endocrinol. 41: 239-49. PMID: 18682532
10. Activating transcription factor 4 and CCAAT/enhancer-binding protein-beta negatively regulate the mammalian target of rapamycin via Redd1 expression in response to oxidative and endoplasmic reticulum stress.  |  Jin, HO., et al. 2009. Free Radic Biol Med. 46: 1158-67. PMID: 19439225
11. Analysis of Il36a induction by C/EBPβ via a half-CRE•C/EBP element in murine macrophages in dependence of its CpG methylation level.  |  Nerlich, A., et al. 2021. Genes Immun. 22: 313-321. PMID: 34697411
12. Coexistence of C/EBP alpha, beta, growth-induced proteins and DNA synthesis in hepatocytes during liver regeneration. Implications for maintenance of the differentiated state during liver growth.  |  Greenbaum, LE., et al. 1995. J Clin Invest. 96: 1351-65. PMID: 7657810
13. DNA binding by C/EBP proteins correlates with hepatocyte proliferation.  |  Soriano, HE., et al. 1995. In Vitro Cell Dev Biol Anim. 31: 703-9. PMID: 8564082
14. Cloning of the novel human myeloid-cell-specific C/EBP-epsilon transcription factor.  |  Chumakov, AM., et al. 1997. Mol Cell Biol. 17: 1375-86. PMID: 9032264
15. Isoform-specific regulation of the CCAAT/enhancer-binding protein family of transcription factors by 3′,5′-cyclic adenosine monophosphate in Sertoli cells.  |  Grønning, LM., et al. 1999. Endocrinology. 140: 835-43. PMID: 9927313