SRE Gel Shift Oligonucleotides: sc-2523

SRE Gel Shift Oligonucleotides are short DNA sequences specifically designed for applications in gel shift assays, a foundational technique in molecular biology used to study protein-DNA interactions. These oligonucleotides are derived from the consensus binding sequence of the serum response element (SRE), a regulatory DNA motif found in the promoters of genes responsive to extracellular signals such as growth factors, cytokines, and stress stimuli. The SRE motif is recognized and bound by transcription factors such as serum response factor (SRF) and ternary complex factors (TCFs), which play key roles in mediating cellular responses to environmental cues and regulating gene expression. By utilizing SRE Gel Shift Oligonucleotides in gel shift assays, researchers can investigate the binding kinetics, specificity, and affinity of SRF and TCFs to SRE motifs under diverse experimental conditions. This technique enables the elucidation of the molecular mechanisms underlying SRF and TCF-mediated gene regulation and provides insights into the complex signaling networks that govern cellular processes such as cell growth, differentiation, and apoptosis.

SRE Gel Shift Oligonucleotides References:

1. Persistent increased DNA-binding and expression of serum response factor occur with epilepsy-associated long-term plasticity changes.  |  Morris, TA., et al. 1999. J Neurosci. 19: 8234-43. PMID: 10493724
2. Cardiomyopathy in transgenic mice with cardiac-specific overexpression of serum response factor.  |  Zhang, X., et al. 2001. Am J Physiol Heart Circ Physiol. 280: H1782-92. PMID: 11247792
3. Early postnatal cardiac changes and premature death in transgenic mice overexpressing a mutant form of serum response factor.  |  Zhang, X., et al. 2001. J Biol Chem. 276: 40033-40. PMID: 11514558
4. Helicobacter pylori CagA protein activates serum response element-driven transcription independently of tyrosine phosphorylation.  |  Hirata, Y., et al. 2002. Gastroenterology. 123: 1962-71. PMID: 12454853
5. Differential patterns of human cytomegalovirus gene expression in various T-cell lines carrying human T-cell leukemia-lymphoma virus type I: role of Tax-activated cellular transcription factors.  |  Beck, Z., et al. 2003. J Med Virol. 71: 94-104. PMID: 12858414
6. Serum response factor is a critical requirement for VEGF signaling in endothelial cells and VEGF-induced angiogenesis.  |  Chai, J., et al. 2004. FASEB J. 18: 1264-6. PMID: 15180964
7. Identification of a novel serum response factor cofactor in cardiac gene regulation.  |  Zhang, X., et al. 2004. J Biol Chem. 279: 55626-32. PMID: 15492011
8. Alternative splicing and nonsense-mediated mRNA decay regulate gene expression of serum response factor.  |  Zhang, X., et al. 2007. Gene. 400: 131-9. PMID: 17629633
9. Angiotensin II regulation of neuromodulation: downstream signaling mechanism from activation of mitogen-activated protein kinase.  |  Lu, D., et al. 1996. J Cell Biol. 135: 1609-17. PMID: 8978826