Myc-Max Gel Shift Oligonucleotides: sc-2509

Myc-Max Gel Shift Oligonucleotides are short DNA sequences specifically designed for use in gel shift assays, a fundamental technique in molecular biology research aimed at studying protein-DNA interactions. These oligonucleotides are derived from the consensus binding sequence of the Myc-Max transcription factor complex, comprising c-Myc and Max proteins. The Myc-Max complex plays a critical role in the regulation of gene expression, particularly genes involved in cell growth, proliferation, differentiation, and apoptosis. Upon dimerization, Myc-Max binds to specific DNA sequences, known as E-box motifs, within the promoters of target genes, thereby modulating their transcriptional activity. By utilizing Myc-Max Gel Shift Oligonucleotides in gel shift assays, researchers can investigate the binding kinetics, specificity, and affinity of the Myc-Max complex to E-box motifs under various experimental conditions. This technique enables the elucidation of the molecular mechanisms underlying Myc-Max-mediated gene regulation and provides insights into the complex signaling networks governing cellular processes such as oncogenesis, development, and differentiation.

Myc-Max Gel Shift Oligonucleotides References:

1. The suppression of ornithine decarboxylase expression and cell proliferation at the promotion stage of lung tumorigenesis in mice by alpha-tocopheryloxybutyric acid.  |  Yano, T., et al. 2001. Biochem Pharmacol. 61: 1177-81. PMID: 11301052
2. Polyamine-modulated expression of c-myc plays a critical role in stimulation of normal intestinal epithelial cell proliferation.  |  Liu, L., et al. 2005. Am J Physiol Cell Physiol. 288: C89-99. PMID: 15355849
3. Association of the mSin3A-histone deacetylase 1/2 corepressor complex with the mouse steroidogenic acute regulatory protein gene.  |  Clem, BF. and Clark, BJ. 2006. Mol Endocrinol. 20: 100-13. PMID: 16109738
4. Comparative genomic and functional analyses reveal a novel cis-acting PTEN regulatory element as a highly conserved functional E-box motif deleted in Cowden syndrome.  |  Pezzolesi, MG., et al. 2007. Hum Mol Genet. 16: 1058-71. PMID: 17341483
5. N-Myc and SP regulate phosphatidylserine synthase-1 expression in brain and glial cells.  |  Tasseva, G., et al. 2011. J Biol Chem. 286: 1061-73. PMID: 21068393