E2F-1 Gel Shift Oligonucleotides: sc-2507

E2F-1 Gel Shift Oligonucleotides are short DNA sequences meticulously designed for applications in gel shift assays, a fundamental technique in molecular biology research used to investigate protein-DNA interactions. These oligonucleotides originate from the consensus binding sequence of the E2F-1 transcription factor, a member of the E2F family known for its pivotal role in cell cycle regulation and transcriptional control. E2F-1 regulates the expression of genes involved in cell proliferation, DNA repair, and apoptosis, thereby influencing fundamental cellular processes. Upon activation, E2F-1 binds to specific DNA sequences, known as E2F binding sites, within the promoters of its target genes, modulating their transcriptional activity. By employing E2F-1 Gel Shift Oligonucleotides in gel shift assays, researchers can explore the binding affinity, specificity, and kinetics of E2F-1 to E2F binding sites under diverse experimental conditions. This technique facilitates the elucidation of the molecular mechanisms underlying E2F-1-mediated gene regulation and provides insights into the signaling networks governing cell cycle progression, proliferation, and cell fate determination.

E2F-1 Gel Shift Oligonucleotides References:

1. The mdm2 proto-oncogene sensitizes human medullary thyroid carcinoma cells to ionizing radiation.  |  Dilla, T., et al. 2002. Oncogene. 21: 2376-86. PMID: 11948421
2. Role of retinoblastoma protein and E2F-1 transcription factor in the acquisition of 5-fluorouracil resistance by colon cancer cells.  |  Obama, K., et al. 2002. Int J Oncol. 21: 309-14. PMID: 12118326
3. Molecular mechanisms of impaired stimulation of DNA synthesis in cultured hepatocytes of aged rats.  |  Kitano, S., et al. 1998. Am J Physiol. 275: C146-54. PMID: 9688845