Oct-1 Gel Shift Oligonucleotides: sc-2506

Oct-1 Gel Shift Oligonucleotides are short DNA sequences designed for use in gel shift assays, a fundamental technique in molecular biology used to study protein-DNA interactions. These oligonucleotides are derived from the consensus binding sequence of the Oct-1 transcription factor, which belongs to the POU (Pit-Oct-Unc) domain family of transcription factors. Oct-1 plays a crucial role in the regulation of gene expression by binding to specific DNA sequences, known as octamer motifs, located within the promoters of target genes. Upon binding to its target sites, Oct-1 can either activate or repress gene transcription, depending on the context and its interaction with other regulatory proteins. By utilizing Oct-1 Gel Shift Oligonucleotides in gel shift assays, researchers can investigate the binding kinetics, specificity, and affinity of Oct-1 to octamer motifs under various experimental conditions. This technique allows for the elucidation of the molecular mechanisms underlying Oct-1-mediated gene regulation and provides insights into the complex signaling networks that govern cellular processes such as development, differentiation, and proliferation.

Oct-1 Gel Shift Oligonucleotides References:

1. Octamer transcription factor-1 enhances hepatic nuclear factor-1alpha-mediated activation of the human UDP glucuronosyltransferase 2B7 promoter.  |  Ishii, Y., et al. 2000. Mol Pharmacol. 57: 940-7. PMID: 10779377
2. Allele-specific binding of the ubiquitous transcription factor OCT-1 to the functional single nucleotide polymorphism (SNP) sites in the tumor necrosis factor-alpha gene (TNFA) promoter.  |  Hohjoh, H. and Tokunaga, K. 2001. Genes Immun. 2: 105-9. PMID: 11393654
3. Binding of octamer factors to the murine IL-5 CLE0 in primary T-cells and a T-cell line.  |  Salerno, MS., et al. 2001. Cytokine. 15: 4-9. PMID: 11509003
4. 3-Hydroxy-3-methylglutaryl coenzyme A reductase inhibitors increase the binding activity and nuclear level of Oct-1 in mononuclear cells.  |  Ortego, M., et al. 2002. Eur J Pharmacol. 448: 113-21. PMID: 12144930
5. Involvement of Oct-1 in transcriptional regulation of beta-casein gene expression in mouse mammary gland.  |  Zhao, FQ., et al. 2002. Biochim Biophys Acta. 1577: 27-37. PMID: 12151092
6. TALE homeodomain proteins regulate gonadotropin-releasing hormone gene expression independently and via interactions with Oct-1.  |  Rave-Harel, N., et al. 2004. J Biol Chem. 279: 30287-97. PMID: 15138251
7. Oct-1 and nuclear factor Y bind to the SURG-1 element to direct basal and gonadotropin-releasing hormone (GnRH)-stimulated mouse GnRH receptor gene transcription.  |  Kam, KY., et al. 2005. Mol Endocrinol. 19: 148-62. PMID: 15388790
8. Triptolide, an active component of the Chinese herbal remedy Tripterygium wilfordii Hook F, inhibits production of nitric oxide by decreasing inducible nitric oxide synthase gene transcription.  |  Wang, B., et al. 2004. Arthritis Rheum. 50: 2995-303. PMID: 15457469
9. Regulation of the rat CYP4A2 gene promoter by c-Jun and octamer binding protein-1.  |  Fiala-Beer, E., et al. 2007. Int J Biochem Cell Biol. 39: 1235-47. PMID: 17481938
10. Association of serum interleukin-6 concentration with a functional IL6 -6331T>C polymorphism.  |  Smith, AJ., et al. 2008. Clin Chem. 54: 841-50. PMID: 18356242
11. Lamin B1 controls oxidative stress responses via Oct-1.  |  Malhas, AN., et al. 2009. J Cell Biol. 184: 45-55. PMID: 19139261
12. A far-upstream Oct-1 motif regulates cytokine-induced transcription of the human inducible nitric oxide synthase gene.  |  Park, KS., et al. 2009. J Mol Biol. 390: 595-603. PMID: 19467240
13. Transcriptional activity of human papillomavirus type 31b enhancer is regulated through synergistic interaction of AP1 with two novel cellular factors.  |  Kyo, S., et al. 1995. Virology. 211: 184-97. PMID: 7645210
14. Oct-1 binds promoter elements required for transcription of the GnRH gene.  |  Eraly, SA., et al. 1998. Mol Endocrinol. 12: 469-81. PMID: 9544983