NFκB Gel Shift Oligonucleotides: sc-2505

NFκB Gel Shift Oligonucleotides are short sequences of DNA that serve as crucial tools in molecular biology research, specifically in the study of transcription factor binding and gene regulation. These oligonucleotides are designed based on the consensus binding sequence of nuclear factor kappa B (NFκB), a key transcription factor involved in the regulation of immune and inflammatory responses, as well as cell survival and proliferation. NFκB is activated in response to various stimuli, including proinflammatory cytokines, pathogens, and cellular stressors, leading to its translocation into the nucleus and subsequent binding to specific DNA sequences known as κB sites within target gene promoters. By utilizing NFκB Gel Shift Oligonucleotides in gel shift assays, researchers can investigate the binding affinity, specificity, and kinetics of NFκB to κB sites under different experimental conditions. This technique enables the elucidation of the molecular mechanisms underlying NFκB-mediated gene transcription and provides insights into the complex signaling pathways that regulate immune and inflammatory responses, as well as other physiological processes.

NFκB Gel Shift Oligonucleotides References:

1. Variation at the von Willebrand factor (vWF) gene locus is associated with plasma vWF:Ag levels: identification of three novel single nucleotide polymorphisms in the vWF gene promoter.  |  Keightley, AM., et al. 1999. Blood. 93: 4277-83. PMID: 10361125
2. IL-13 activates STAT6 and inhibits liver injury induced by ischemia/reperfusion.  |  Yoshidome, H., et al. 1999. Am J Pathol. 155: 1059-64. PMID: 10514388
3. Inhibition of lipopolysaccharide-mediated NFkappaB activation by ethanol in human monocytes.  |  Mandrekar, P., et al. 1999. Int Immunol. 11: 1781-90. PMID: 10545482
4. Anti-inflammatory gene therapy directed at the airway epithelium.  |  Griesenbach, U., et al. 2000. Gene Ther. 7: 306-13. PMID: 10694811
5. Interrelations between nuclear-factor kappa B activation, glial response and neuronal apoptosis in gerbil hippocampus after ischemia.  |  Domańska-Janik, K., et al. 2001. Acta Neurobiol Exp (Wars). 61: 45-51. PMID: 11315321
6. Antigen-specific suppression of a primed immune response by dendritic cells mediated by regulatory T cells secreting interleukin-10.  |  Martin, E., et al. 2003. Immunity. 18: 155-67. PMID: 12530984
7. HIV-1 Tat directly binds to NFkappaB enhancer sequence: role in viral and cellular gene expression.  |  Dandekar, DH., et al. 2004. Nucleic Acids Res. 32: 1270-8. PMID: 14981150
8. Down-regulation by nuclear factor kappaB of human 25-hydroxyvitamin D3 1alpha-hydroxylase promoter.  |  Ebert, R., et al. 2004. Mol Endocrinol. 18: 2440-50. PMID: 15243130
9. Induction of Raf kinase inhibitor protein contributes to macrophage differentiation.  |  Schuierer, MM., et al. 2006. Biochem Biophys Res Commun. 342: 1083-7. PMID: 16513087
10. Up regulation of GW112 Gene by NF kappaB promotes an antiapoptotic property in gastric cancer cells.  |  Kim, KK., et al. 2010. Mol Carcinog. 49: 259-70. PMID: 19908244
11. Rottlerin inhibits ROS formation and prevents NFkappaB activation in MCF-7 and HT-29 cells.  |  Maioli, E., et al. 2009. J Biomed Biotechnol. 2009: 742936. PMID: 20168983
12. A herbal formula containing roots of Salvia miltiorrhiza (Danshen) and Pueraria lobata (Gegen) inhibits inflammatory mediators in LPS-stimulated RAW 264.7 macrophages through inhibition of nuclear factor κB (NFκB) pathway.  |  Cheung, DW., et al. 2013. J Ethnopharmacol. 145: 776-83. PMID: 23261483
13. Manipulation of distinct NFkappaB proteins alters interleukin-1beta-induced human rheumatoid synovial fibroblast prostaglandin E2 formation.  |  Roshak, AK., et al. 1996. J Biol Chem. 271: 31496-501. PMID: 8940164
14. Inhibition of NFkappaB-mediated interleukin-1beta-stimulated prostaglandin E2 formation by the marine natural product hymenialdisine.  |  Roshak, A., et al. 1997. J Pharmacol Exp Ther. 283: 955-61. PMID: 9353419