CREB Gel Shift Oligonucleotides: sc-2504

CREB Gel Shift Oligonucleotides are short DNA sequences designed for use in gel shift assays, a fundamental technique in molecular biology for studying protein-DNA interactions. These oligonucleotides are derived from the consensus binding sequence of the cAMP response element binding protein (CREB), a transcription factor known for its role in cellular signaling and gene expression regulation. CREB is activated by phosphorylation in response to various extracellular stimuli, including neurotransmitters, hormones, and growth factors, and subsequently binds to specific DNA sequences known as cAMP response elements (CREs) within target gene promoters. By utilizing CREB Gel Shift Oligonucleotides in gel shift assays, researchers can investigate the binding kinetics, specificity, and affinity of CREB to CRE sequences under different experimental conditions. This technique allows for the elucidation of the molecular mechanisms underlying CREB-mediated transcriptional regulation and provides insights into the complex signaling networks that govern cellular responses to environmental stimuli.

CREB Gel Shift Oligonucleotides References:

1. The 3′,5′-cyclic adenosine monophosphate response element binding protein (CREB) is functionally reduced in human toxic thyroid adenomas.  |  Brunetti, A., et al. 2000. Endocrinology. 141: 722-30. PMID: 10650954
2. NF-kappa B regulates the LPS-induced expression of interleukin 12 p40 in murine peritoneal macrophages: roles of PKC, PKA, ERK, p38 MAPK, and proteasome.  |  Zhang, JS., et al. 2000. Cell Immunol. 204: 38-45. PMID: 11006016
3. Gene expression of catecholamine biosynthetic enzymes following exercise: modulation by age.  |  Tυmer, N., et al. 2001. Neuroscience. 103: 703-11. PMID: 11274789
4. A new polymorphic site located in the human UCP1 gene controls the in vitro binding of CREB-like factor.  |  Rousset, S., et al. 2002. Int J Obes Relat Metab Disord. 26: 735-8. PMID: 12032762
5. Diminished transcription factor survival signals in dorsal root ganglia in rats with streptozotocin-induced diabetes.  |  Purves, TD. and Tomlinson, DR. 2002. Ann N Y Acad Sci. 973: 472-6. PMID: 12485914
6. cAMP elevators inhibit LPS-induced IL-12 p40 expression by interfering with phosphorylation of p38 MAPK in murine peritoneal macrophages.  |  Feng, WG., et al. 2002. Cell Res. 12: 331-7. PMID: 12528891
7. Adenosine stimulates CREB activation in macrophages via a p38 MAPK-mediated mechanism.  |  Nεmeth, ZH., et al. 2003. Biochem Biophys Res Commun. 312: 883-8. PMID: 14651954
8. Cell type-specific bidirectional regulation of the glucocorticoid-induced leucine zipper (GILZ) gene by estrogen.  |  Tynan, SH., et al. 2004. J Steroid Biochem Mol Biol. 91: 225-39. PMID: 15336700
9. Regulation of cyclin D2 and the cyclin D2 promoter by protein kinase A and CREB in lymphocytes.  |  White, PC., et al. 2006. Oncogene. 25: 2170-80. PMID: 16301994
10. Akt-and CREB-mediated prostate cancer cell proliferation inhibition by Nexrutine, a Phellodendron amurense extract.  |  Garcia, GE., et al. 2006. Neoplasia. 8: 523-33. PMID: 16820098
11. Identification of a biphasic signaling pathway involved in ischemic resistance of the hippocampal dentate gyrus.  |  Blanquet, PR., et al. 2006. Exp Neurol. 202: 357-72. PMID: 16996500
12. Pivotal role of connective tissue growth factor in lung fibrosis: MAPK-dependent transcriptional activation of type I collagen.  |  Ponticos, M., et al. 2009. Arthritis Rheum. 60: 2142-55. PMID: 19565505
13. Restless legs syndrome-associated intronic common variant in Meis1 alters enhancer function in the developing telencephalon.  |  Spieler, D., et al. 2014. Genome Res. 24: 592-603. PMID: 24642863
14. Two CGTCA motifs and a GHF1/Pit1 binding site mediate cAMP-dependent protein kinase A regulation of human growth hormone gene expression in rat anterior pituitary GC cells.  |  Shepard, AR., et al. 1994. J Biol Chem. 269: 1804-14. PMID: 8294429
15. CREB controls LAP/C/EBP beta transcription.  |  Niehof, M., et al. 1997. Mol Cell Biol. 17: 3600-13. PMID: 9199295