DAB, 50X (CAS 7411-49-6)

Usage :

For Immunohistochemical:

1. To 50 parts of Tris hydrochloride solution, pH 7.6 (SC-301951), add 1 part of DAB, 50X and 1 part of 0.5% hydrogen peroxide aqueous solution. Mix well and protect from light.

1a. Hydrogen peroxide, 0.5%, prepared from 30% hydrogen peroxide (sc-203336) as follows: Dilute 0.167mL of 30% hydrogen peroxide to 10 mL with reagent grade water.

2. Completely cover tissue sections with this solution and incubate 5 - 15 minutes at room temperature.

3. After reaction is complete, wash sections thoroughly in reagent grade water.

4. Counterstain with hemotoxylin (sc-24973) if desired.

5. Dehydrate in graded alcohols to xylene or xylene substitutes.

6. Mount using xylene based mounting media (e.g., sc-45087).

For Blotting:

1. To 50 parts of Tris/maleic acid buffer, add 1 part of DAB Concentrate and 1 part of 0.5% hydrogen peroxide aqueous solution. Mix well and protect from light.

1a. Hydrogen peroxide, 0.5%, prepared from 30% hydrogen peroxide (sc-203336) as follows: Dilute 0.167mL of 30% hydrogen peroxide to 10 mL with reagent grade water.

1b. Tris/Maleic acid buffer prepared as follows: Dissolve 12.1g of tris base (sc-3715) in approximately 800mL of reagent grade water. Adjust pH to 6.0 with a 2 M solution of maleic acid (prepared with sc-250287). QS to 1 L with reagent grade water.

2. Completely cover membranes with this solution and incubate 5 - 30 minutes at room temperature.

3. After reaction is complete, wash membranes thoroughly in reagent quality water.

4. Air dry and store protected from light.

* Discard if there is precipitate in the solution prior to mixing with hydrogen peroxide. Discard if reagent is still purple after making solution with hydrogen peroxide.

Formulation :

50X concentrate

Appearance :

Solution

Physical State :

Liquid

Solubility :

Soluble in water (Dilutable). Insoluble in most organic solvents.

Storage :

Store at 4° C

Refractive Index :

n²⁰_D 1.77 (Predicted)

PubChem CID :

MDL Number :

MFCD00012969

EC Number :

231-018-9

SMILES :

C1=CC(=C(C=C1C2=CC(=C(C=C2)N)N)N)N.Cl.Cl.Cl.Cl