DAB, 50X (CAS 7411-49-6)

5 5 (Based on popularity) 1

Synonym:	3,3' Diaminobenzidine Tetrahydrochloride
Application:	A buffer suitable for HRP-based immunoblotting and immunohistochemical assays
CAS Number:	7411-49-6
Molecular Weight:	360.1
Molecular Formula:	C₁₂H₁₄N₄ 4HCl

Ordering Information

Product Name	Catalog #	Unit	Price	Qty	Add
DAB, 50X	sc-24982	10 ml	$75
DAB, 50X	sc-24982A	100 ml	$500

Description

DAB (3-3′ diaminobenzidine tetrachloride) is suitable for HRP-based immunoblotting and immunohistochemical assays. Oxidizes to a brown polymer in the presence of HRP and hydrogen peroxide that is insoluble in most organic solvents; sites of HRP activity on tissue sections and blots appear as brown-orange deposits, which can be intensified by treatment with salts of silver, copper, nickel, cobalt and osmium.

Usage

Dilute to 1x with 100mM Tris-HCl pH 7.6
Recommended for use with UltraCruz™ Mounting Medium (sc-24941).
Discard if a precipitate forms or if reagent is purple (the purple material is a DAB oxidation product that binds avidly to heme-containing proteins).

Technical Information

Formulation:	50X concentrate
Appearance:	Dark brown solution
Physical State:	Liquid
Solubility:	Soluble in water (Dilutable). Insoluble in most organic solvents.
Storage:	Store at 4° C
Refractive Index:	n²⁰_D 1.77 (Predicted)

Safety and Reference Information

PubChem CID:	23892
MDL Number:	MFCD00012969
EC Number:	231-018-9
SMILES:	NC1=C(N)C=C(C=C1)C1=CC(N)=C(N)C=C1.Cl.Cl.Cl.Cl

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

References

1. Krueger, S.K., et al. 1999. Biotech. Histochem. 74: 105-109. PMID: 10333408
2. Gallyas, F., et al. 1982. J. Histochem. Cytochem. 30: 183-184. PMID: 7061820
3. Larrson, L.I. 1988. Immunocytochemistry: Theory and Practice. Boca Raton, FL. CRC Press, Inc.

DAB, 50X (CAS 7411-49-6) Product Citations

See how others have used DAB, 50X (CAS 7411-49-6).

24 total citations

Loading citations.

SDS

Datasheet/SDS