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- Specifications
- Offered as 10 ml (sc-24982)
- Synonym: 3,3' Diaminobenzidine Tetrahydrochloride
- CAS Number: 7411-49-6
- Molecular Weight: 360.1
- Molecular Formula: C12H14N4 ·4HCl
- Form: Liquid
- Physical Appearance: dark brown solution
- Solubility: Insoluble in most organic solvents
- Concentration: 50X concentrate
- SMILES: NC1=C(N)C=C(C=C1)C1=CC(N)=C(N)C=C1.Cl.Cl.Cl.Cl
- Description
- DAB (3-3′ diaminobenzidine tetrachloride) is suitable for HRP-based immunoblotting and immunohistochemical assays. Oxidizes to a brown polymer in the presence of HRP and hydrogen peroxide that is insoluble in most organic solvents; sites of HRP activity on tissue sections and blots appear as brown-orange deposits, which can be intensified by treatment with salts of silver, copper, nickel, cobalt and osmium.
- Storage
- Store at 4° C. Discard if a precipitate forms or if reagent is purple (the purple material is a DAB oxidation product that binds avidly to heme-containing proteins).
- Usage
- Dilute to 1x with 100mM Tris-HCl pH 7.6
Recommended for use with Clarion Mounting Medium (sc-24942).
Discard if a precipitate forms or if reagent is purple (the purple material is a DAB oxidation product that binds avidly to heme-containing proteins).
- Safety
- UN Number: UN 2929, Class 3 (6.1), Packing group II
- Risk Statements: R40
- Safety Statements: S36,S37
- For Research Use Only
- Reference Numbers
- PubChem Compound ID: 23892
- MDL Number: MFCD00012969
- EC Number: 231-018-9
- References
- 1. Krueger, S.K., et al. 1999. Biotech. Histochem. 74: 105-109. PMID: 10333408
2. Gallyas, F., et al. 1982. J. Histochem. Cytochem. 30: 183-184. PMID: 7061820
3. Larrson, L.I. 1988. Immunocytochemistry: Theory and Practice. Boca Raton, FL. CRC Press, Inc.
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